Developing and Characterizing a Biocompatible Hydrogel Obtained by Cross-Linking Gelatin with Oxidized Sodium Alginate for Potential Biomedical Applications

The main goal of this research was to create biocompatible hydrogels using gelatin and a double cross-linking technique involving both covalent and ionic bonds to immobilize propolis. The covalent bonds were formed through Schiff base cross-links between protein-free amino groups (NH₂) from the lysine residue and aldehyde groups (CHO) produced by oxidizing sodium alginate with NaIO₄, while the ionic bonds were achieved using Mg²⁺ ions. Hydrogel films were obtained by varying the molar ratios of -CHO/-NH₂ under different pH conditions (3.5 and 5.5). The presence of aldehyde groups in the oxidized sodium alginate (OSA) was confirmed using FTIR and NMR spectroscopy. The oxidation degree was monitored over 48 h, and the influence of temperature was examined. Results showed that higher -CHO/-NH₂ molar ratios led to increased conversion index values of NH₂ groups, and a decrease in swelling degree values was observed in mediums with pH values of 5.5 and 7.4. The encapsulation and release efficiency of propolis decreased with an increase in the hydrogel cross-linking degree. UV irradiation enhanced the antioxidant activity of both free and encapsulated propolis. These findings offer valuable insights for the biomedical and pharmaceutical fields into designing biocompatible hydrogels for propolis immobilization, with potential for controlled release.