A comprehensive two-hybrid analysis to explore the <i>Legionella pneumophila</i> effector–effector interactome
Secreted bacterial effector proteins are typically viewed as modulators of host activity, entering the host cytosol to physically interact with and modify the activity of one or more host proteins in support of infection. A growing body of evidence suggests that a subset of effectors primarily function to modify the activities of other effectors inside the host. These "effectors of effectors" or metaeffectors are often identified through experimental serendipity during the study of canonical effector function against the host. We previously performed the first global effector-wide genetic interaction screen for metaeffectors within the arsenal of <i>Legionella pneumophila</i>, an intracellular bacterial pathogen with over 300 effectors. Here, using a high-throughput, scalable methodology, we present the first global interaction network of physical interactions between <i>L. pneumophila</i> effectors. This data set serves as a complementary resource to identify and understand both the scope and nature of non-canonical effector activity within this important human pathogen.
Authors
- Atina G. Coté (ORCID: https://orcid.org/0000-0002-0340-9325)
- Jennifer J. Knapp (ORCID: https://orcid.org/0000-0003-3347-4686)
- Michael A. Calderwood (ORCID: https://orcid.org/0000-0001-6475-1418)
- Morgan O. Petersen (ORCID: https://orcid.org/0009-0002-2708-0090)
- Marc Vidal (ORCID: https://orcid.org/0000-0003-3391-5410)
- Alexander W. Ensminger (ORCID: https://orcid.org/0000-0003-0824-3704)
- Kerstin Spirohn (ORCID: https://orcid.org/0000-0002-2071-1606)
- Florent Laval (ORCID: https://orcid.org/0000-0001-7744-6199)
- Nishka Kishore (ORCID: https://orcid.org/0000-0002-9219-4310)
- Malene L. Urbanus (ORCID: https://orcid.org/0009-0008-0850-3805)
- Georges Coppin (ORCID: https://orcid.org/0000-0001-7647-3240)
- Dae‐Kyum Kim (ORCID: https://orcid.org/0000-0003-4568-8278)
- Dayag Sheykhkarimli (ORCID: https://orcid.org/0000-0001-8415-6659)
- Jean‐Claude Twizere (ORCID: https://orcid.org/0000-0002-8683-705X)
- Harley O’Connor Mount (ORCID: https://orcid.org/0000-0003-1401-9178)
- Roujia Li
- Frederick P. Roth (ORCID: https://orcid.org/0000-0002-6628-649X)
Institutions
- Harvard University (US)
- University of Liège (BE)
- The University of Melbourne (AU)
- University of Pittsburgh (US)
- University of Toronto (CA)
- Lunenfeld-Tanenbaum Research Institute (CA)
- Gembloux Agro-Bio Tech (BE)
- Donnelly College (US)
- Peter Doherty Institute (AU)
- Boston VA Research Institute (US)
- Dana-Farber Cancer Institute (US)
Publication Details
- Journal
- mSystems
- Published
- 2024-11-11
- DOI
- https://doi.org/10.1128/msystems.01004-24
- Citations
- 6
- Primary Topic
- Legionella and Acanthamoeba research
- Type
- article
- Field-Weighted Citation Impact
- 3.70
Funders
- Fonds De La Recherche Scientifique - FNRS
- Université de Liège
- Fonds Léon Fredericq
- Wallonie-Bruxelles International
- Canadian Institutes of Health Research
- Natural Sciences and Engineering Research Council of Canada