Adenine base editors induce off-target structure variations in mouse embryos and primary human T cells
This study shows both CRISPR/Cas9 and ABE induce off-target SVs in mouse embryos and primary human T cells, raising an urgent need for the development of high-fidelity gene editing tools.
Authors
- Pinzheng Huang
- Yingqi Li (ORCID: https://orcid.org/0000-0002-2300-3023)
- Tanglong Yuan (ORCID: https://orcid.org/0000-0003-4670-2625)
- Erwei Zuo (ORCID: https://orcid.org/0000-0001-8259-0275)
- Changyang Zhou (ORCID: https://orcid.org/0000-0002-3012-1001)
- Yidi Sun (ORCID: https://orcid.org/0000-0002-4191-2917)
- Leilei Wu (ORCID: https://orcid.org/0000-0003-1814-5745)
- Yaqin Li (ORCID: https://orcid.org/0000-0003-2363-4834)
- Shutan Jiang
- Marie B. Shi (ORCID: https://orcid.org/0000-0003-4214-2069)
Institutions
- Agricultural Genomics Institute at Shenzhen (CN)
- Center for Excellence in Brain Science and Intelligence Technology (CN)
- Institute of Modern Physics (CN)
- Chinese Academy of Agricultural Sciences (CN)
- Ministry of Agriculture and Rural Affairs (CN)
- University of Chinese Academy of Sciences (CN)
Publication Details
- Journal
- Genome biology
- Published
- 2024-11-11
- DOI
- https://doi.org/10.1186/s13059-024-03434-0
- Citations
- 14
- Primary Topic
- CAR-T cell therapy research
- Type
- article
- Field-Weighted Citation Impact
- 3.96
Funders
- National Natural Science Foundation of China
- Chinese Academy of Agricultural Sciences