Enrichment, Antioxidant and Enzyme Inhibition Activities of Flavonoids from <i>Artemisia Selengensis</i> Turcz.

Macroporous resin was used to enrich flavonoids in the ethyl acetate extract of Artemisia Selengensis Turcz. Based on a single factor experiment, the enrichment process was optimized using the response surface method. The optimal parameters of the enrichment process were a sample concentration of 0.3 mg/mL, a loading rate of 1 mL/min, an elution flow rate of 2 mL/min, and a total flavonoid content of 155.38±0.97 mg/g. The flavonoids enriched by AB-8 macroporous resin demonstrated significant scavenging activities against DPPH, ABTS⁺, and hydroxyl free radicals, and also exhibited certain inhibitory effects on α-amylase and α-glucosidase. Among them, the scavenging ability of the flavonoids enriched by AB-8 macroporous resin on hydroxyl free radical (IC₅₀=30.31±1.92 μg/mL) was the closest to Vc, and the inhibitory effect on α-glucosidase (IC₅₀=16.19±1.35 μg/mL) was the best. These findings confirmed the potential of Artemisia Selengensis Turcz. was a natural antioxidant and hypoglycemic drug.