DNA methylation analysis of multiple genes in thymic epithelial tumors
<b>Aim:</b> To investigate DNA methylation levels of a panel of genes in thymic epithelial tumors (TETs).<b>Materials & methods:</b> We selected 15 genes among the most promising epigenetic biomarkers of TETs and evaluated their methylation levels in 71 TET samples.<b>Results:</b> thymic carcinomas (TCs) showed hypermethylation of <i>GHSR</i> and <i>ELF3</i> genes and reduced <i>IL1RN</i> methylation levels compared with thymomas (TMs) and healthy thymic tissues. <i>RAG1</i> was hypomethylated in TMs compared with healthy thymic tissues. No difference in the methylation levels of the investigated genes was seen among TM stages and subtypes. No changes in blood methylation levels of the investigated genes were seen among TET subtypes.<b>Conclusion:</b> The present study confirms <i>GHSR, ELF3, IL1RN</i> and <i>RAG1</i> as TET epigenetic biomarkers.
Authors
- Marco Lucchi (ORCID: https://orcid.org/0000-0001-9909-6820)
- Iacopo Petrini (ORCID: https://orcid.org/0000-0002-7752-6866)
- Franca Melfi (ORCID: https://orcid.org/0000-0002-1773-8269)
- Fabio Coppedè (ORCID: https://orcid.org/0000-0002-3081-6647)
- Andrea Stoccoro (ORCID: https://orcid.org/0000-0002-6768-8466)
- Eleonora Pardini (ORCID: https://orcid.org/0000-0003-1285-601X)
- Diana Bacchin (ORCID: https://orcid.org/0000-0002-5706-0974)
- Melania Guida (ORCID: https://orcid.org/0000-0003-1968-5874)
- Lucia Migliore
- Michelangelo Maestri (ORCID: https://orcid.org/0000-0003-0960-4938)
- Vittorio Aprile (ORCID: https://orcid.org/0000-0001-8538-1719)
- Vanessa Nicolì (ORCID: https://orcid.org/0000-0003-2751-7352)
- Roberta Ricciardi (ORCID: https://orcid.org/0009-0006-9506-0904)
- Martina Lari
- Martin Giangreco
Institutions
- University of Pisa (IT)
- Azienda Ospedaliera Universitaria Pisana (IT)
Publication Details
- Journal
- Epigenomics
- Published
- 2024-11-08
- DOI
- https://doi.org/10.1080/17501911.2024.2419362
- Primary Topic
- Myasthenia Gravis and Thymoma
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Regione Toscana