New record of Carnidae (Diptera) from Taiwan and potential challenges in DNA barcode amplification due to pseudogene

The following new information regarding <i>C.orientalis</i> is provided in this study: <i>Carnusorientalis</i> is first recorded in Taiwan, filling the gap in its East Asian distribution. This is also the first record of Carnidae from Taiwan.<i>Otuslettia</i> (Hodgson, 1836) (Aves, Strigidae) is reported as a new host for <i>C.orientalis</i>, identified on a fallen fledgling.Co-amplification of the host's <i>COI</i> is reported in this study using the universal PCR primer set LCO1490/HCO2198. Additionally, the amplification of a <i>COI</i>-like pseudogene using a newly-designed primer set is detected through abnormal translated amino acid sequences and the occurrence of a stop codon.New specific primers for the <i>COI</i> gene of <i>Carnus</i> were designed in this study. The new distribution and ecological data of <i>C.orientalis</i> enhance our understanding of this species. The provision of new <i>COI</i> primers is anticipated to contribute to future studies employing DNA barcoding in bird-parasitic flies.