Aristolochia of the Siphisia Section In Vitro

The results of this study represent an optimization protocol for clonal micropropagation of four species of the genus Aristolochia, section Siphisia. Plants of these species are rare, endemic, and medicinal plants. 6-Benzylaminopurine (BAP) (0.5, 1.0, 1.2 and 1.4 mg/L) and indolylbutyric acid (IBA) (0.5 and 1.0 mg/L) were used in this work. The use of subcultivation with different nutrient media (MS supplemented with 0.5 mg/L BAP at the propagation stage and 1/2 WPM with the addition of 0.5 mg/L IBA at the rooting stage) contributes to the mass production of microplants. The maximum values of the microshoot height for each species were detected on the medium with 0.5 mg/L BAP. Explants of A. macrophylla and A. manshuriensis were characterized with the greatest reproduction coefficient (34 pcs.), while A. californica explants displayed the smallest coefficient (18.6 pcs.). The difference is associated with the activation of a different number of buds in each species.