Generation of two isogenic human iPSC lines (ZIPi013-B-1, ZIPi013-B-2) carrying a CRISPR/Cas9-mediated deletion of TRPM4
Two isogenic hiPSC lines, ZIPi013-B-1 and ZIPi013-B-2, were generated by CRISPR/Cas9-mediated indels in the TRPM4 gene of the previously published ZIPi013-B. TRPM4 belongs to the evolutionarily conserved family of transient receptor potential (TRP) channels. It is expressed ubiquitously and its activity is regulated by intracellular calcium binding, changes in membrane potential, phosphoinositide lipids in the plasma membrane and the local concentration of cytoplasmic ATP and ADP. TRPM4 has been implicated in various diseases, including neurological and immune system disorders, cardiac diseases and cancer. Both new cell lines offer the opportunity to model human diseases and test therapeutic modalities addressing these.
Authors
- Dennis S.C. Lam (ORCID: https://orcid.org/0000-0001-5276-3702)
- Manuel Alexander Friese (ORCID: https://orcid.org/0000-0001-6380-2420)
- William H. Schaefer
- Narasimha Swamy Telugu (ORCID: https://orcid.org/0000-0003-4509-7056)
- Sebastian Diecke (ORCID: https://orcid.org/0000-0002-5219-5992)
- Undine Haferkamp (ORCID: https://orcid.org/0000-0003-2893-5847)
- Lars Binkle-Ladisch (ORCID: https://orcid.org/0000-0001-6168-3210)
- Ole Pleß (ORCID: https://orcid.org/0000-0002-1468-316X)
- Kim Krieg (ORCID: https://orcid.org/0009-0006-5231-5401)
Institutions
- Universität Hamburg (DE)
- Max Delbrück Center (DE)
- Fraunhofer Institute for Translational Medicine and Pharmacology (DE)
- University Medical Center Hamburg-Eppendorf (DE)
- Institute of Neuroimmunology of the Slovak Academy of Sciences (SK)
Publication Details
- Journal
- Stem Cell Research
- Published
- 2024-11-08
- DOI
- https://doi.org/10.1016/j.scr.2024.103609
- Primary Topic
- CRISPR and Genetic Engineering
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Deutsche Forschungsgemeinschaft
- Bundesministerium für Bildung und Forschung