Bacterial flotillins as destabilizers of phospholipid membranes
From archaea to mammals evolutionary conserved flotillins are scaffolding proteins, recognized for their nandomain-segregating activity. Flotillins form basket-like oligomeric architectures on the membrane, based on a conserved secondary structure composition of the monomeric subunits: a membrane-targeting region, an SPFH domain and a coiled-coil "flotillin" domain. In B. subtilis, the two flotillins FloT and FloA are present, localizing mainly in distinct nanodomains and executing multiple cellular functions. We here use deuterium and phosphorus solid-state NMR to monitor the effect of the different flotillins FloT and FloA and their structural components on model membranes. We find a clear disordering effect of FloT and FloA on the membranes reaching the carbon positions in the centre of the membrane. This effect is imposed by the hydrophobic region and the adjacent SPFH domain and, surprisingly, further supported by the membrane-distant flotillin domain. Biological implications of this disordering action are discussed.
Authors
- Estelle Morvan (ORCID: https://orcid.org/0000-0003-4071-7885)
- Marc Bramkamp (ORCID: https://orcid.org/0000-0002-7704-3266)
- Abigail Savietto Scholz
- Dênis Martinez (ORCID: https://orcid.org/0000-0002-3578-0372)
- Érick J. Dufourc (ORCID: https://orcid.org/0000-0002-8221-9326)
- Mélanie Berbon (ORCID: https://orcid.org/0000-0003-3235-253X)
- Ana Álvarez‐Mena (ORCID: https://orcid.org/0000-0002-5357-8944)
- Axelle Grélard (ORCID: https://orcid.org/0000-0002-6708-2253)
- Birgit Habenstein (ORCID: https://orcid.org/0000-0003-3723-9552)
- Sarah Turpin
Institutions
- Centre National de la Recherche Scientifique (FR)
- Université de Bordeaux (FR)
- Inserm (FR)
- Hochschule für Angewandte Wissenschaften Kiel (DE)
- Christian-Albrechts-Universität zu Kiel (DE)
- Chimie et Biologie des Membranes et des Nanoobjects (FR)
- Institut Européen de Chimie et Biologie (FR)
- Institut Polytechnique de Bordeaux (FR)
Publication Details
- Journal
- Biochimica et Biophysica Acta (BBA) - Biomembranes
- Published
- 2024-11-08
- DOI
- https://doi.org/10.1016/j.bbamem.2024.184399
- Citations
- 6
- Primary Topic
- Caveolin-1 and cellular processes
- Type
- article
- Field-Weighted Citation Impact
- 1.96
Funders
- Deutsche Forschungsgemeinschaft
- Agence Nationale de la Recherche
- Institut National de la Santé et de la Recherche Médicale
- Centre National de la Recherche Scientifique