Self-reference digital holography unit adaptable to optical microscope with light-emitting diode

To perform holographic three-dimensional (3D) imaging and quantitative phase imaging with daily-use light, I developed a self-reference digital holography unit that is adaptable to an optical microscope with a light-emitting diode. The developed unit is combined with a commercially available optical microscope to implement a speckleless digital holographic microscope. Holographic 3D imaging of an amplitude object and phase objects and simultaneous imaging of fluorescence intensity and quantitative phase distributions are experimentally demonstrated with the unit.