The role of the essential GTPase ObgE in regulating lipopolysaccharide synthesis in Escherichia coli

During growth, cells need to synthesize and expand their envelope, a process that requires careful regulation. Here, we show that the GTPase ObgE of E. coli contributes to the regulation of lipopolysaccharide (LPS) synthesis, an essential component of the Gram-negative outer membrane. Using a dominant-negative mutant (named 'ObgE*'), we show a direct interaction between ObgE and LpxA, which catalyzes the first step in LPS synthesis. This interaction is enhanced by the mutation in ObgE* which, when bound to GTP, leads to inhibition of LpxA, decreased LPS synthesis, and cell death. Although wild-type ObgE does not exert the same strong effects as ObgE* on LpxA or LPS synthesis, our data indicate that ObgE participates in the regulation of cell envelope synthesis in E. coli. Because ObgE also influences other cellular functions (i.e., ribosome assembly, DNA replication, etc.), it seems increasingly plausible that this GTPase coordinates several processes to finetune cell growth.

Authors

Institutions

Publication Details

Journal
Nature Communications
Published
2024-11-08
DOI
https://doi.org/10.1038/s41467-024-53980-1
Citations
4
Primary Topic
Bacterial Genetics and Biotechnology
Type
article
Field-Weighted Citation Impact
2.20

Funders

Controls
|||
ALL TIME
JAN
FEB
MAR
APR
MAY
JUN
JUL
AUG
SEP
OCT
article

The role of the essential GTPase ObgE in regulating lipopolysaccharide synthesis in Escherichia coli

Anna‐Maria Möller, Sibylle C. Vonesch, Israel Mares‐Mejía, Babette Deckers et al.
4 citations
Nature Communications
Bacterial Genetics and Biotechnology
2.20
article

The role of the essential GTPase ObgE in regulating lipopolysaccharide synthesis in Escherichia coli

Anna‐Maria Möller, Sibylle C. Vonesch, Israel Mares‐Mejía, Babette Deckers, Wim Versées, Silke Vercauteren, Paul Matthay, Liselot Dewachter, Franz Narberhaus, Simon Brückner, Elen Louwagie, J.-F. Michiels
article en
4 citations

Abstract

During growth, cells need to synthesize and expand their envelope, a process that requires careful regulation. Here, we show that the GTPase ObgE of E. coli contributes to the regulation of lipopolysaccharide (LPS) synthesis, an essential component of the Gram-negative outer membrane. Using a dominant-negative mutant (named 'ObgE*'), we show a direct interaction between ObgE and LpxA, which catalyzes the first step in LPS synthesis. This interaction is enhanced by the mutation in ObgE* which, when bound to GTP, leads to inhibition of LpxA, decreased LPS synthesis, and cell death. Although wild-type ObgE does not exert the same strong effects as ObgE* on LpxA or LPS synthesis, our data indicate that ObgE participates in the regulation of cell envelope synthesis in E. coli. Because ObgE also influences other cellular functions (i.e., ribosome assembly, DNA replication, etc.), it seems increasingly plausible that this GTPase coordinates several processes to finetune cell growth.

Nature CommunicationsVol. 15(1)
Ablynx (Belgium) (BE), Vrije Universiteit Brussel (BE), de Duve Institute (BE), VIB-KU Leuven Center for Microbiology (BE), VIB-VUB Center for Structural Biology (BE), Ruhr University Bochum (DE), UCLouvain (BE), KU Leuven (BE)
Deutsche Forschungsgemeinschaft, Fonds De La Recherche Scientifique - FNRS, Fonds Wetenschappelijk Onderzoek, KU Leuven, Vrije Universiteit Brussel, Vlaamse regering
Openalex Percentile: Top 11%
Bacterial Genetics and Biotechnology
2.20
DOIOpen Access PDFOpenAlex
AI Navigator

Ask Laika to Summarize, Analyze, and Connect papers live on the map.

Summarize Papers & Methodologies

Extract key findings, datasets, and comparative methods across publications.

Benchmark Rankings & Visual Analytics

Rank top research institutions, authors, funders, topics, and journals by Field-Weighted Citation Impact (FWCI) and paper volume with instant charts.

Connect Distant Disciplines

Bridge topological clusters on the map to find hidden collaborative intersections.