Establishment and application of highly efficient regeneration, genetic transformation and genome editing system for cucurbitacins biosynthesis in Hemsleya chinensis
Here, we established a process for regeneration, transformation, and genome editing of H. chinensis using stem segments. This provides valuable insight into the underlying molecular mechanisms of medicinal compound production. By combining high-efficiency tissue culture, transformation, and genome editing systems, we provide a powerful platform that supports functional research on molecular mechanisms of secondary metabolism.
Authors
- Guanghui Zhang (ORCID: https://orcid.org/0000-0002-1905-6069)
- Lesong Li
- Sheng-Chao Yang
- Chun-Fan Xiang
- Yan Zhao (ORCID: https://orcid.org/0000-0001-8088-4288)
- Xia Li (ORCID: https://orcid.org/0000-0002-9794-2648)
- Chaohui Li (ORCID: https://orcid.org/0000-0001-7893-9665)
- Pinhan Zhou
- Juan Wang (ORCID: https://orcid.org/0000-0002-2531-2188)
Institutions
- Yunnan Agricultural University (CN)
Publication Details
- Journal
- BMC Plant Biology
- Published
- 2024-11-07
- DOI
- https://doi.org/10.1186/s12870-024-05717-x
- Citations
- 3
- Primary Topic
- CRISPR and Genetic Engineering
- Type
- article
- Field-Weighted Citation Impact
- 0.70
Funders
- National Natural Science Foundation of China
- China Agricultural University
- South China Agricultural University