Effect of probiotics on the rumen microbiome of lactating cows

Methods for isolating metagenomic DNA from the rumen fluid of dairy cows have been selected and have made it possible to obtain high-purity nucleic acid preparations without PCR inhibitors. The probiotic potential of feed additives “Sporobact-K” and “Rumibact” based on spore-forming bacteria of the genus Bacillus and the propionic acid bacteria of the genus Propionibacterium were assessed. The feasibility of their use to increase resistance to opportunistic microbiota, improve ruminal digestion and control acidosis in cows was shown. The dominant taxa of the rumen microbiome of dairy cows were determined, and the primers and temperature-time parameters were selected for their detection by PCR. The feasibility of using feed additives “Sporobact-K”, based on bacteria of the genus Bacillus and “Rumibact”, based on propionic acid bacteria to control opportunistic microbiota, improve scar digestion and reduce the risk of acidosis in lactating cows is shown.