Cordycepin generally inhibits growth factor signal transduction in a systems pharmacology study

Cordycepin (3' deoxyadenosine) has been widely researched as a potential cancer therapy, but many diverse mechanisms of action have been proposed. Here, we confirm that cordycepin triphosphate is likely to be the active metabolite of cordycepin and that it consistently represses growth factor-induced gene expression. Bioinformatic analysis, quantitative PCR and western blotting confirmed that cordycepin blocks the PI3K/AKT/mTOR and/or MEK/ERK pathways in six cell lines and that AMPK activation is not required. The effects of cordycepin on translation through mTOR pathway repression were detectable within 30 min, indicating a rapid process. These data therefore indicate that cordycepin has a universal mechanism of action, acting as cordycepin triphosphate on an as yet unknown target molecule involved in growth factor signalling.

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Publication Details

Journal
FEBS Letters
Published
2024-11-07
DOI
https://doi.org/10.1002/1873-3468.15046
Citations
2
Primary Topic
Medicinal Plants and Bioactive Compounds
Type
article
Field-Weighted Citation Impact
0.47

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article

Cordycepin generally inhibits growth factor signal transduction in a systems pharmacology study

Keith A. Spriggs, Jialiang Lin, Graeme J. Thorn, Sadaf Ashraf et al.
2 citations
FEBS Letters
Medicinal Plants and Bioactive Compounds
0.47
article

Cordycepin generally inhibits growth factor signal transduction in a systems pharmacology study

Keith A. Spriggs, Jialiang Lin, Graeme J. Thorn, Sadaf Ashraf, Steven Lawrence, Cornelia H. de Moor, David A. Barrett, Wahyu Utami, Richa Singhania, Irengbam Rocky Mangangcha, Asma Khurshid, Dong‐Hyun Kim
article en
2 citations

Abstract

Cordycepin (3' deoxyadenosine) has been widely researched as a potential cancer therapy, but many diverse mechanisms of action have been proposed. Here, we confirm that cordycepin triphosphate is likely to be the active metabolite of cordycepin and that it consistently represses growth factor-induced gene expression. Bioinformatic analysis, quantitative PCR and western blotting confirmed that cordycepin blocks the PI3K/AKT/mTOR and/or MEK/ERK pathways in six cell lines and that AMPK activation is not required. The effects of cordycepin on translation through mTOR pathway repression were detectable within 30 min, indicating a rapid process. These data therefore indicate that cordycepin has a universal mechanism of action, acting as cordycepin triphosphate on an as yet unknown target molecule involved in growth factor signalling.

FEBS LettersVol. 599(3)
University of Nottingham (GB), Sunny BioDiscovery (United States) (US)
University of Dundee, University of Leicester, University of Delhi, Versus Arthritis, Medical Research Council, Biotechnology and Biological Sciences Research Council
Good health and well-being
Openalex Percentile: Top 34%
Medicinal Plants and Bioactive Compounds
0.47
DOIOpen Access PDFOpenAlex
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