Fungal Extracellular Enzymes from Aspergillus spp. as Promising Candidates for Extra-Heavy Oil Degradation and Enhanced Oil Recovery

Heavy crude oil (HCO) and extra-heavy crude oil (EHCO) with high viscosity and density pose enormous challenges to the exploitation of oil reserves. While bacteria are increasingly used in biocatalytic upgrading of HCO and EHCO, less attention has been paid to the potential of fungi. The aim of this study was to ascertain the role of fungal extracellular enzymes from <i>Aspergillus</i> spp. In the biodegradation of EHCO and their application potential for enhanced oil recovery. <i>A. terreus</i> HJ2 and <i>A. nidulans</i> HJ4 with the ability to biodegrade HCO were previously isolated from bitumen enrichment cultures. Both strains grew well on EHCO agar plates supplemented with a small amount of soluble starch (0.2%) and yeast extract (0.3%). Extracellular enzymes from each strain separately, as well as mixtures of the enzymes, exhibited EHCO degradation activity, leading to redistribution of hydrocarbons with substantial formation of biogases and organic acids in a 7-day period. Enzymatic degradation resulted in decreased contents of resins and asphaltenes, accompanied by increased contents of saturates and aromatics. Gas chromatography-mass spectrometry revealed distinct redistribution patterns of <i>n</i>-alkane in the biotreated oil. Enzymatic degradation additionally caused considerable reduction in oil viscosity (by 12.7%) and heavy metal concentrations (Ni, by 44.1%; Fe, by 54.0%; V, by 31.6%). The results provide empirical evidence for the application potential of fungal extracellular enzymes from <i>Aspergillus</i> spp. in EHCO recovery and biocatalytic upgrading of EHCO.