Extracellular vesicles of different cellular origin feature distinct biomolecular corona dynamics
Initially observed on synthetic nanoparticles, the existence of biomolecular corona and its role in determining nanoparticle identity and function are now beginning to be acknowledged in biogenic nanoparticles, particularly in extracellular vesicles - membrane-enclosed nanoparticle shuttling proteins, nucleic acids, and metabolites which are released by cells for physiological and pathological communication - we developed a methodology based on fluorescence correlation spectroscopy to track biomolecular corona formation on extracellular vesicles derived from human red blood cells and amniotic membrane mesenchymal stromal cells when these vesicles are dispersed in human plasma. The methodology allows for tracking corona dynamics <i>in situ</i> under physiological conditions. Results evidence that the two extracellular vesicle populations feature distinct corona dynamics. These findings indicate that the dynamics of the biomolecular corona may ultimately be linked to the cellular origin of the extracellular vesicles, revealing an additional level of heterogeneity, and possibly of bionanoscale identity, that characterizes circulating extracellular vesicles.
Authors
- Paolo di Gianvincenzo
- Paolo Bergese (ORCID: https://orcid.org/0000-0002-4652-2168)
- Angelo Musicò (ORCID: https://orcid.org/0000-0001-6193-4366)
- Miriam Romano (ORCID: https://orcid.org/0000-0002-3972-460X)
- Lucia Paolini (ORCID: https://orcid.org/0000-0002-4410-5272)
- Annalisa Radeghieri (ORCID: https://orcid.org/0000-0003-2737-1090)
- Sergio Enrique Moya (ORCID: https://orcid.org/0000-0002-7174-1960)
- Marina Cretich (ORCID: https://orcid.org/0000-0001-8251-5275)
- Antonietta Rosa Silini (ORCID: https://orcid.org/0000-0001-6208-3583)
- Andrea Papait (ORCID: https://orcid.org/0000-0003-1229-9671)
- Arabella Neva (ORCID: https://orcid.org/0000-0002-2593-3785)
- Ornella Parolini (ORCID: https://orcid.org/0000-0002-5211-6430)
- Valentina Mangolini (ORCID: https://orcid.org/0000-0002-5548-7405)
- Santiago Giménez Reyes
- Camillo Almici (ORCID: https://orcid.org/0000-0002-9438-2569)
- Andrea Zendrini (ORCID: https://orcid.org/0000-0002-3626-0360)
Institutions
- Università Cattolica del Sacro Cuore (IT)
- Consejo Nacional de Investigaciones Científicas y Técnicas (AR)
- Agostino Gemelli University Polyclinic (IT)
- CIC biomaGUNE (ES)
- Instituto de Física del Sur (AR)
- Consorzio Interuniversitario per lo Sviluppo dei Sistemi a Grande Interfase (IT)
- Azienda Socio Sanitaria Territoriale degli Spedali Civili di Brescia (IT)
- Fondazione Poliambulanza Istituto Ospedaliero (IT)
- National Research Council (IT)
- National Interuniversity Consortium of Materials Science and Technology (IT)
- Istituto di Scienze e Tecnologie Chimiche "Giulio Natta" (IT)
- University of Brescia (IT)
Publication Details
- Journal
- Nanoscale Horizons
- Published
- 2024-11-07
- DOI
- https://doi.org/10.1039/d4nh00320a
- Citations
- 17
- Primary Topic
- Extracellular vesicles in disease
- Type
- article
- Field-Weighted Citation Impact
- 3.97
Funders
- Università degli Studi di Milano
- Consorzio Interuniversitario Nazionale per la Scienza e Tecnologia dei Materiali
- European Commission
- Ministero dell’Istruzione, dell’Università e della Ricerca
- Ministerio de Ciencia e Innovación
- Horizon 2020 Framework Programme
- Horizon 2020