Transport-of-intensity phase imaging using commercially available confocal microscope
TIE-based quantitative phase imaging (QPI) using commercially available confocal microscopy is proposed. We evaluated the feasibility of the proposed method by measuring the microlens array, plant, and mammalian cultured cells. The experimental result indicates that QPI can be realized in commercially available confocal microscopy using the TIE technique. This method will be useful for measuring dry mass, viscosity, and temperature of cells and for correcting phase fluctuation to cancel aberration and scattering caused by an object in the future.
Authors
- Yosuke Tamada (ORCID: https://orcid.org/0000-0002-4845-4867)
- Takumi Tomoi (ORCID: https://orcid.org/0000-0002-9407-0426)
- Naru Yoneda (ORCID: https://orcid.org/0000-0001-9964-5420)
- Tomomi Nemoto (ORCID: https://orcid.org/0000-0001-6102-1495)
- Osamu Matoba (ORCID: https://orcid.org/0000-0001-9767-0059)
- Joe Sakamoto (ORCID: https://orcid.org/0000-0003-2295-0659)
Institutions
- Tokyo University of Science (JP)
- National Institutes of Natural Sciences (JP)
- The Graduate University for Advanced Studies, SOKENDAI (JP)
- Utsunomiya University (JP)
- National Institute for Physiological Sciences (JP)
- The Exploratory Research Center on Life and Living Systems (JP)
- Kobe University (JP)
Publication Details
- Journal
- Journal of Biomedical Optics
- Published
- 2024-11-07
- DOI
- https://doi.org/10.1117/1.jbo.29.11.116002
- Citations
- 2
- Primary Topic
- Digital Holography and Microscopy
- Type
- article
- Field-Weighted Citation Impact
- 0.68
Funders
- Japan Agency for Medical Research and Development
- Japan Society for the Promotion of Science
- National Institute for Basic Biology