Virulence effectors encoded in the rice yellow dwarf phytoplasma genome participate in pathogenesis

Bacteria-like phytoplasmas alternate between plant and insect hosts, secreting proteins that disrupt host development. In this study, we sequenced the complete genome of "Candidatus Phytoplasma oryzae" strain HN2022, associated with rice yellow dwarf (RYD) disease, using PacBio HiFi technology. The strain was classified within the 16Sr XI-B subgroup. Through SignalP v5.0 for prediction and subsequent expression analysis of secreted proteins in Nicotiana benthamiana and rice (Oryza sativa L.), we identified the key virulence effector proteins RY348 and RY378. RY348, a homolog of Secreted Aster Yellows Phytoplasma Effector 54 (SAP54), targets and degrades the MADS-box transcription factors MADS1 and MADS15, causing pollen sterility. Meanwhile, RY378 impacts the strigolactone and auxin signaling pathways, substantially increasing tillering. These findings offer insights into the interactions between plants and phytoplasmas.

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Publication Details

Journal
PLANT PHYSIOLOGY
Published
2024-11-07
DOI
https://doi.org/10.1093/plphys/kiae601
Citations
6
Primary Topic
Phytoplasmas and Hemiptera pathogens
Type
article
Field-Weighted Citation Impact
3.60
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article

Virulence effectors encoded in the rice yellow dwarf phytoplasma genome participate in pathogenesis

Jianguo Wu, Y. S. Zhu, Tianxin Tang, Shuai Zhang et al.
6 citations
PLANT PHYSIOLOGY
Phytoplasmas and Hemiptera pathogens
3.60
article

Virulence effectors encoded in the rice yellow dwarf phytoplasma genome participate in pathogenesis

Jianguo Wu, Y. S. Zhu, Tianxin Tang, Shuai Zhang, Zhihong Zhu, Jisen Zhang, Shanshan Zhao, Zhongkai Zhang, Jie Hu, H. Xie, Qun Hu, Peng Gan, Qiong Hu, Hongxin Guan, C. Li
article en
6 citations

Abstract

Bacteria-like phytoplasmas alternate between plant and insect hosts, secreting proteins that disrupt host development. In this study, we sequenced the complete genome of "Candidatus Phytoplasma oryzae" strain HN2022, associated with rice yellow dwarf (RYD) disease, using PacBio HiFi technology. The strain was classified within the 16Sr XI-B subgroup. Through SignalP v5.0 for prediction and subsequent expression analysis of secreted proteins in Nicotiana benthamiana and rice (Oryza sativa L.), we identified the key virulence effector proteins RY348 and RY378. RY348, a homolog of Secreted Aster Yellows Phytoplasma Effector 54 (SAP54), targets and degrades the MADS-box transcription factors MADS1 and MADS15, causing pollen sterility. Meanwhile, RY378 impacts the strigolactone and auxin signaling pathways, substantially increasing tillering. These findings offer insights into the interactions between plants and phytoplasmas.

PLANT PHYSIOLOGYVol. 197(1)
Fujian Normal University (CN), Guangxi University (CN), Rice Research Institute (CN), Fujian Academy of Agricultural Sciences (CN), Guangxi Academy of Agricultural Science (CN), Yunnan Academy of Agricultural Sciences (CN), State Key Laboratory For Conservation and Utilization of Subtropical Agro-Bioresources (CN), Fujian Agriculture and Forestry University (CN)
Life in Land
Openalex Percentile: Top 7%
Phytoplasmas and Hemiptera pathogens
3.60
DOISourceOpenAlex
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