Transparent and Mechanically Robust Janus Nanofiber Membranes for Open Wound Healing and Monitoring

The electrospun nanofiber membrane has demonstrated great potential for wound management due to its porous structure, large surface area, mechanical strength, and barrier properties. However, there is a need to develop transparent bioactive nanofibers with strong mechanical properties to facilitate the monitoring of the healing process. In this study, we present an electrospinning-based method for creating transparent (∼80-90%), strong (∼11-13 MPa), and Janus nanofiber membranes. The innovative square pattern architecture of the membrane includes a thin hydrophobic polycaprolactone layer on top of a layer of hydrophilic ethylene-vinyl alcohol nanofiber, which enables the absorption of excess biofluid from the wound and exhibits Janus wettability for water. Furthermore, incorporating 5% chitosan into the composition of the nanofibers accelerates the healing process through its antioxidant properties and antimicrobial activity against various bacteria, including drug-resistant strains. The developed membrane also demonstrates skin-repairing function, quick blood clotting (around 145 ± 12 s), and biocompatibility with keratinocyte (≥90%), as well as <i>in vitro</i> quick cell migration (∼24 h). With a tensile strength of 11-13 MPa, the membrane effectively adheres to the knee joint even after running 4 km. These optimal properties of the electrospun nanofiber membrane make it suitable for effective wound management and inspection of the healing process, without the need for frequent dressing changes.