Molecular and morphological characterisation of Ditylenchoides anyae sp. n. (Nematoda: Anguinoidea) from Iran

Summary A new species, Ditylenchoides anyae sp. n., recovered from soil in the headland areas of non-cultivated fields in Alborz Province, Iran, is described and illustrated based on its morphological, morphometric, and molecular characters. The new species was characterised by a slender body and length of 784 (663-925) μ m (females) and 667-712 μ m (males), lip region continuous, stylet length 6.4 (6.0-7.0) μ m, lateral fields with four incisures, and excretory pore situated 110 (82-137) μ m from the anterior end, median bulb of pharynx fusiform, non-muscular, and non-valvate, isthmus elongate, slender ending at a pyriform basal pharyngeal bulb not overlapping intestine, vulva with distinct and relatively large lateral flaps, a well-developed post-vulval uterine sac, 31.3 (27-35) μ m long, a female tail elongate-conoid with pointed terminus; and male with adanal bursa and spicules 21-22 μ m long. The new species was characterised using the D2-D3 expansion segments of 28S rRNA, ITS rRNA, and partial 18S rRNA gene sequences. Phylogenetic analyses of the genus Ditylenchoides are given based on the analysis of these genes.