Identification of cambium stem cell factors and their positioning mechanism
Wood constitutes the largest reservoir of terrestrial biomass. Composed of xylem, it arises from one side of the vascular cambium, a bifacial stem cell niche that also produces phloem on the opposing side. It is currently unknown which molecular factors endow cambium stem cell identity. Here we show that TRACHEARY ELEMENT DIFFERENTIATION INHIBITORY FACTOR (TDIF) ligand-activated PHLOEM INTERCALATED WITH XYLEM (PXY) receptors promote the expression of CAMBIUM-EXPRESSED AINTEGUMENTA-LIKE (CAIL) transcription factors to define cambium stem cell identity in the <i>Arabidopsis</i> root. By sequestrating the phloem-originated TDIF, xylem-expressed PXY confines the TDIF signaling front, resulting in the activation of CAIL expression and stem cell identity in only a narrow domain. Our findings show how signals emanating from cells on opposing sides ensure robust yet dynamically adjustable positioning of a bifacial stem cell layer.
Authors
- Jingyi Han (ORCID: https://orcid.org/0000-0003-1106-2461)
- Alexis Porcher (ORCID: https://orcid.org/0000-0002-8966-4203)
- David Dolan (ORCID: https://orcid.org/0000-0002-3610-0340)
- J. Ortiz (ORCID: https://orcid.org/0009-0000-7488-0993)
- Gugan Eswaran (ORCID: https://orcid.org/0000-0002-3439-3696)
- Hiroyuki Iida (ORCID: https://orcid.org/0000-0001-7311-0700)
- Jing Zhang (ORCID: https://orcid.org/0000-0003-4463-7516)
- Marina Leal Gavarrón (ORCID: https://orcid.org/0000-0002-2879-1516)
- Leo Vainio (ORCID: https://orcid.org/0000-0002-3305-2531)
- Jacob Pieter Rutten (ORCID: https://orcid.org/0000-0003-0369-7205)
- Siobhán M. Brady (ORCID: https://orcid.org/0000-0001-9424-8055)
- Riikka Mäkilä (ORCID: https://orcid.org/0000-0002-8547-0452)
- Kirsten ten Tusscher (ORCID: https://orcid.org/0000-0002-1945-7858)
- Melis Kücükoglu (ORCID: https://orcid.org/0000-0002-2655-7398)
- Brecht Wybouw (ORCID: https://orcid.org/0000-0001-8783-4646)
- J. Peter Etchells (ORCID: https://orcid.org/0000-0002-8524-4949)
- Ondřej Smetana (ORCID: https://orcid.org/0000-0002-0511-7713)
- Tiina Blomster (ORCID: https://orcid.org/0000-0001-7113-1604)
- Ari Pekka Mähönen (ORCID: https://orcid.org/0000-0001-6051-866X)
- Xin Wang (ORCID: https://orcid.org/0000-0003-0582-5362)
- Benjamin Planterose Jiménez (ORCID: https://orcid.org/0000-0001-8985-9572)
- Xixi Zhang (ORCID: https://orcid.org/0000-0001-7048-4627)
Institutions
- University of Helsinki (FI)
- Durham University (GB)
- Utrecht University (NL)
- University of California, Davis (US)
Publication Details
- Journal
- Science
- Published
- 2024-11-07
- DOI
- https://doi.org/10.1126/science.adj8752
- Citations
- 21
- Primary Topic
- Plant Molecular Biology Research
- Type
- article
- Field-Weighted Citation Impact
- 12.59
Funders
- Helsingin Yliopisto
- European Commission
- Nederlandse Organisatie voor Wetenschappelijk Onderzoek
- Biotechnology and Biological Sciences Research Council
- Japan Society for the Promotion of Science