A canonical protein complex controls immune homeostasis and multipathogen resistance
The calcium (Ca<sup>2+</sup>) sensor ROD1 (RESISTANCE OF RICE TO DISEASES1) is a master regulator of immunity in rice. By screening suppressors of <i>rod1</i> mutants, we show that ROD1 governs immune homeostasis by surveilling the activation of a canonical immune pathway. Mutations in <i>OsTIR</i> (<i>TIR-only protein</i>), <i>OsEDS1</i> (<i>enhanced disease susceptibility 1</i>), <i>OsPAD4</i> (<i>phytoalexin deficient 4</i>), and <i>OsADR1</i> (<i>activated disease resistance 1</i>) all abolish enhanced disease resistance of <i>rod1</i> plants. OsTIR catalyzes the production of second messengers 2'-(5″-phosphoribosyl)-5'-adenosine monophosphate (pRib-AMP) and diphosphate (pRib-ADP), which trigger formation of an OsEDS1-OsPAD4-OsADR1 (EPA) immune complex. ROD1 interacts with OsTIR and inhibits its enzymatic activity, whereas mutation of <i>ROD1</i> leads to constitutive activation of the EPA complex. Thus, we unveil an immune network that fine-tunes immune homeostasis and multipathogen resistance in rice.
Authors
- Zian Ye
- Shijia Huang (ORCID: https://orcid.org/0000-0002-3715-2986)
- Kui Li (ORCID: https://orcid.org/0000-0001-8790-6419)
- Jane E. Parker (ORCID: https://orcid.org/0000-0002-4700-6480)
- Yue Wu (ORCID: https://orcid.org/0009-0004-6801-9899)
- Mingjun Gao (ORCID: https://orcid.org/0000-0002-2616-915X)
- Yiwen Deng (ORCID: https://orcid.org/0000-0002-6074-5704)
- Jijie Chai (ORCID: https://orcid.org/0000-0001-7591-3873)
- Guoyan Zhao (ORCID: https://orcid.org/0000-0001-5615-6774)
- Xiangbin Zhong (ORCID: https://orcid.org/0000-0002-3938-2486)
- Xiaoyu Chang (ORCID: https://orcid.org/0009-0006-9597-1599)
- Ziyao Lei (ORCID: https://orcid.org/0000-0003-4667-875X)
- Junli Wang (ORCID: https://orcid.org/0000-0001-8825-3894)
- Yonggang Meng (ORCID: https://orcid.org/0000-0001-7551-6325)
- Jiyun Liu (ORCID: https://orcid.org/0000-0002-5352-5199)
- Yu Zhang (ORCID: https://orcid.org/0000-0002-1778-8389)
- Zuhua He (ORCID: https://orcid.org/0000-0002-6098-7893)
- Haochen Zhang (ORCID: https://orcid.org/0009-0006-6950-5244)
- Yuan‐Yuan Gao (ORCID: https://orcid.org/0000-0002-7045-0221)
- Hui Lin (ORCID: https://orcid.org/0009-0002-0642-7761)
- Yuandong Wang (ORCID: https://orcid.org/0000-0001-9072-2143)
- He Yang
- Xin Yin (ORCID: https://orcid.org/0000-0002-8926-5800)
- Weiying Xu (ORCID: https://orcid.org/0009-0000-4895-8064)
- Xin Wang (ORCID: https://orcid.org/0000-0002-6229-1945)
Institutions
- Chinese Academy of Sciences (CN)
- Fudan University (CN)
- ShanghaiTech University (CN)
- Westlake University (CN)
- Zhengzhou University (CN)
- Center for Excellence in Molecular Plant Sciences (CN)
- Max Planck Institute for Plant Breeding Research (DE)
- University of Chinese Academy of Sciences (CN)
- Henan Normal University (CN)
- Zhejiang University (CN)
Publication Details
- Journal
- Science
- Published
- 2024-11-07
- DOI
- https://doi.org/10.1126/science.adr2138
- Citations
- 54
- Primary Topic
- Endoplasmic Reticulum Stress and Disease
- Type
- article
- Field-Weighted Citation Impact
- 17.61