Novel Less Toxic, Lymphoid Tissue‐Targeted Lipid Nanoparticles Containing a Vitamin B5‐Derived Ionizable Lipid for mRNA Vaccine Delivery
Following their approval by the Food and Drug Administration, lipid nanoparticles (LNPs) have emerged as promising tools for delivering mRNA vaccines and therapeutics. Ionizable lipids are among the essential components of LNPs, as they play crucial roles in encapsulating mRNA and facilitating its release into the cytosol. In this study, 17 innovative ionizable lipids using vitamin B5 are designed as the core structure, aiming to reduce toxicity, to maintain vaccine efficiency, and to ensure synthetic feasibility. The top-performing LNP in terms of mRNA vaccine delivery in the mouse model is LNP 5097, which is generated by incorporating ionizable lipid I97. mRNA⊂LNP 5097 demonstrates favorable structural and physicochemical properties, high mRNA transfection efficiency, and long-term stability. Moreover, mRNA⊂LNP 5097 specifically delivers the mRNA to the spleen and lymph nodes in model mice, induces balanced Th1/Th2 immune responses, and elicits the production of high levels of neutralizing antibodies with low toxicity. The findings here suggest the high utility of LNP 5097, which includes novel vitamin B5-derived ionizable lipids with reduced toxicity, in mRNA vaccine research for both infectious diseases and cancer.
Authors
- Eun‐Kyoung Bang (ORCID: https://orcid.org/0000-0002-0339-0740)
- Sung Pil Kwon (ORCID: https://orcid.org/0000-0002-2828-8291)
- Soyeon Yoo (ORCID: https://orcid.org/0000-0002-5734-2517)
- Jae‐Hwan Nam (ORCID: https://orcid.org/0000-0002-0974-4343)
- Seo‐Hyeon Bae (ORCID: https://orcid.org/0000-0002-9037-5361)
- Hyo‐Jung Park (ORCID: https://orcid.org/0000-0002-1601-7638)
- Hyeong Jun Kim (ORCID: https://orcid.org/0000-0003-1742-1349)
- Gyochang Keum (ORCID: https://orcid.org/0000-0001-8767-8022)
- Kounghwa Youn (ORCID: https://orcid.org/0009-0008-9022-2008)
- Muhammad Faisal (ORCID: https://orcid.org/0000-0001-9181-9262)
- In Sung Hwang (ORCID: https://orcid.org/0000-0001-5572-8670)
- Jisun Lee (ORCID: https://orcid.org/0009-0001-5226-4900)
Institutions
- Korea University (KR)
- Kyung Hee University (KR)
- Korea Institute of Brain Science (KR)
- Bio-Medical Science (South Korea) (KR)
- Korea Institute of Science and Technology (KR)
- Catholic University of Korea (KR)
- Korea University of Science and Technology (KR)
Publication Details
- Journal
- Advanced Healthcare Materials
- Published
- 2024-11-06
- DOI
- https://doi.org/10.1002/adhm.202403366
- Citations
- 10
- Primary Topic
- RNA Interference and Gene Delivery
- Type
- article
- Field-Weighted Citation Impact
- 2.34
Funders
- National Research Foundation
- Ministry of Food and Drug Safety
- Korea Institute of Science and Technology
- National Research Foundation of Korea
- Ministry of Science and ICT, South Korea
- National Institutes of Health