Impact of the Stress Response on Quaternary Ammonium Compound Disinfectant Susceptibility in Serratia Species

The well-known problem of antibiotic resistance foreshadows a similar threat posed by microbial resistance to biocides such as disinfectants and antiseptics. These products are vital for infection control, yet their overuse during the COVID-19 pandemic has accelerated the development of resistant microorganisms. This study investigates the molecular mechanisms underlying disinfectant resistance in <i>Serratia</i> sp. HRI. The transcriptomic responses of <i>Serratia</i> sp. HRI were used to identify significant gene expression changes during exposure to QACs and revealed increased methionine transport and polyamine synthesis. Polyamines, crucial in cellular stress responses, were notably upregulated, suggesting a pivotal role of the stress response in disinfectant resistance. Further, our susceptibility tests revealed a marked decrease in susceptibility to QACs under various stress conditions, supporting the hypothesis that stress responses, mediated by polyamines, decrease susceptibility to QACs. This research highlights polyamines as key players in disinfectant resistance, offering novel insights into resistance mechanisms and antimicrobial susceptibility. Our findings emphasise the need for continued investigation into disinfectant resistance and the role of stress responses, particularly polyamine-mediated mechanisms, to direct strategies for preserving disinfectant efficacy and developing future antimicrobial agents.