Characterisation of a Mesophilic <i>Aeromonas salmonicida</i> and the Development of a <scp>PCR</scp> to Differentiate Atypical and Typical Strains

This study describes the identification and characterisation of a new mesophilic Aeromonas salmonicida strain, named HMes1 isolated from Atlantic salmon (Salmo salar L.) in Tasmania. Isolates were identified as Aeromonas salmonicida through phenotypic, phylogenetic and genetic characterisation. After characterisation, the diagnostic phenotypic identification system MicroSys A24 was updated and a new multiplex conventional PCR was developed to enable rapid and inexpensive identification of atypical A. salmonicida, and exclusion of the exotic strain, A. salmonicida ssp. salmonicida.

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Publication Details

Journal
Journal of Fish Diseases
Published
2024-11-06
DOI
https://doi.org/10.1111/jfd.14028
Primary Topic
Aquaculture disease management and microbiota
Type
article
Field-Weighted Citation Impact
0.00

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article

Characterisation of a Mesophilic Aeromonas salmonicida and the Development of a PCR to Differentiate Atypical and Typical Strains

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Journal of Fish Diseases
Aquaculture disease management and microbiota
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Characterisation of a Mesophilic Aeromonas salmonicida and the Development of a PCR to Differentiate Atypical and Typical Strains

Teresa Wilson, Matthew J. Neave, Vivianne Dunn, David Cummins, Mark Green
article en

Abstract

This study describes the identification and characterisation of a new mesophilic Aeromonas salmonicida strain, named HMes1 isolated from Atlantic salmon (Salmo salar L.) in Tasmania. Isolates were identified as Aeromonas salmonicida through phenotypic, phylogenetic and genetic characterisation. After characterisation, the diagnostic phenotypic identification system MicroSys A24 was updated and a new multiplex conventional PCR was developed to enable rapid and inexpensive identification of atypical A. salmonicida, and exclusion of the exotic strain, A. salmonicida ssp. salmonicida.

Journal of Fish DiseasesVol. 48(1)
Commonwealth Scientific and Industrial Research Organisation (AU), Australian Centre for Disease Preparedness (AU)
Commonwealth Scientific and Industrial Research Organisation, Department of Primary Industries and Regional Development, Government of Western Australia
Life below water
Openalex Percentile: Top 75%
Aquaculture disease management and microbiota
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