Modification of the hi-c technology for molecular genetic analysis of formalin-fixed paraffin-embedded sections of tumor tissues
Molecular genetic analysis of tumor tissues is the most important step towards understanding the mechanisms of cancer development, and it is also necessary for the choice of targeted therapy. Hi-C (high-throughput chromatin conformation capture) technology can be used to detect various types of genomic variants, including balanced chromosomal rearrangements: inversions and translocations. In this work, we propose a modification of the Hi-C method for the analysis of chromatin contacts in formalin-fixed paraffin-embedded (FFPE) sections of tumor tissues. Our results demonstrate that this protocol allow to generate high-quality Hi-C data and detect all types of chromosomal rearrangements. We have analyzed various databases to compile a comprehensive list of translocations that hold clinical importance for targeted therapy selection. The practical value of molecular genetic testing is its ability to influence patient treatment strategies and provide prognostic insights. Detecting specific chromosomal rearrangements can guide the choice of targeted therapies, which is a critical aspect of personalized medicine in oncology.
Authors
- Sergey Vladimirovich Vtorushin (ORCID: https://orcid.org/0000-0002-1195-4008)
- Nikita Torgunakov (ORCID: https://orcid.org/0000-0003-2493-0731)
- Maria Gridina (ORCID: https://orcid.org/0000-0002-7972-5949)
- Evgeny V. Denisov (ORCID: https://orcid.org/0000-0003-2923-9755)
- Miroslav Nuriddinov (ORCID: https://orcid.org/0000-0002-2417-8263)
- Veniamin Fishman (ORCID: https://orcid.org/0000-0002-5573-3100)
- Timofey Lagunov (ORCID: https://orcid.org/0000-0003-1966-5910)
- А. I. Ryabova (ORCID: https://orcid.org/0000-0002-7171-8728)
- М. А. Травин (ORCID: https://orcid.org/0000-0001-6385-5784)
- Tatiana S. Gerashchenko (ORCID: https://orcid.org/0000-0002-7283-0092)
- N. V. Vasiliev (ORCID: https://orcid.org/0000-0002-4066-2688)
- Artem A. Shadsky
- М. А. Королев (ORCID: https://orcid.org/0000-0002-4890-0847)
- Ya. K. Stepanchuk
Institutions
- Russian Academy of Sciences (RU)
- Novosibirsk State University (RU)
- Siberian State Medical University (RU)
- Institute of Cytology and Genetics (RU)
- Ministry of Health of the Russian Federation (RU)
- Tomsk National Research Medical Center (RU)
- Institute of Clinical and Experimental Lymphology (RU)
Publication Details
- Journal
- Биохимия
- Published
- 2024-11-06
- DOI
- https://doi.org/10.31857/s0320972524040042
- Primary Topic
- Molecular Biology Techniques and Applications
- Type
- article
- Field-Weighted Citation Impact
- 0.00