Acidification-based mineral weathering mechanism involves a glucose/methanol/choline oxidoreductase in <i>Caballeronia mineralivorans</i> PML1(12)
This work deciphers the molecular and genetic bases used by strain PML1(12) of <i>Caballeronia mineralivorans</i> to weather minerals. Through bioinformatics analyses, we identified a total of four GMC-FAD oxidoreductases in the genome of strain PML1(12) and a putative PQQ-dependent glucose dehydrogenase. Through a combination of physiological and geochemical analyses, we revealed that one of them (i.e., GMC3) was the enzyme responsible for the acidification-based mineral weathering mechanism used by strain PML1(12). To date, a single representative of this enzyme family has been identified in the effective mineral-weathering bacterial strain <i>Collimonas pratensis</i> PMB3(1). Phylogenetic analyses revealed that this new system appeared conserved in the <i>Collimonas</i> genus. The new findings presented in this work demonstrate that GMC oxidoreductases can have an active role in other effective MWe bacteria outside of collimonads and that <i>Caballeronia</i> are capable of weathering minerals using this type of enzyme. Our findings offer relevant information for different fields of research, such as environmental genomics, microbiology, chemistry, evolutionary biology, and soil sciences.
Authors
- Carine Cochet
- Laura Picard
- Stéphane Uroz (ORCID: https://orcid.org/0000-0001-9412-7210)
- Philippe Oger (ORCID: https://orcid.org/0000-0001-6298-6870)
- Cédric Paris (ORCID: https://orcid.org/0000-0001-8673-3130)
- Cintia Blanco Nouche
- Marie-Pierre Turpault
Institutions
- Université Claude Bernard Lyon 1 (FR)
- Centre National de la Recherche Scientifique (FR)
- Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (FR)
- Ecologie fonctionnelle & biogéochimie des sols & des agro-systèmes (FR)
- Interactions Arbres-Microorganismes (FR)
- Ecologie et Ecophysiologie Forestières (FR)
- École Nationale Supérieure Agronomique (DZ)
- Laboratoire des Biomolécules (FR)
- Laboratoire d'Ingénierie des Biomolécules
- Institut National des Sciences Appliquées de Lyon (FR)
- Université de Lorraine (FR)
Publication Details
- Journal
- Applied and Environmental Microbiology
- Published
- 2024-11-06
- DOI
- https://doi.org/10.1128/aem.01221-24
- Citations
- 5
- Primary Topic
- Botanical Research and Applications
- Type
- article
- Field-Weighted Citation Impact
- 1.74
Funders
- Indian National Science Academy
- Recherches Avancées sur la Biologie de l’Arbre et les Ecosystèmes Forestiers
- Agence Nationale de la Recherche
- Centre National de la Recherche Scientifique
- Université Claude Bernard Lyon 1
- Institut National des Sciences Appliquées de Lyon
- Conseil Régional de Lorraine
- Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement