Antisenescence Expansion of Mesenchymal Stem Cells Using Piezoelectric β-Poly(vinylidene fluoride) Film-Based Culture
Regenerative therapies based on mesenchymal stem cells (MSCs) show promise in treating a wide range of disorders. However, the replicative senescence of MSCs during <i>in vitro</i> expansion poses a challenge to obtaining a substantial quantity of high-quality MSCs. In this investigation, a piezoelectric β-poly(vinylidene fluoride) film-based culture plate (β-CP) was developed with an antisenescence effect on cultured human umbilical cord-derived MSCs. Compared to traditional tissue culture plates (TCPs) and α-poly(vinylidene fluoride) film-based culture plates, the senescence markers of p21, p53, interleukin-6 and insulin-like growth factor-binding protein-7, stemness markers of OCT4 and NANOG, and telomere length of MSCs cultured on β<i>-</i>CPs were significantly improved. Additionally, MSCs at passage 18 cultured on β-CPs showed significantly better multipotency and pro-angiogenic capacities in vitro, and higher wound healing abilities in a mouse model. Mechanistically, β-CPs rejuvenated senescent MSCs by improving mitochondrial functions and mitigating oxidative and glycoxidative stresses. Overall, this study presents β-CPs as a promising approach for efficient and straightforward antisenescence expansion of MSCs while preserving their stemness, thereby holding great potential for large-scale production of MSCs for clinical application in cell therapies.
Authors
- Jiao Qu (ORCID: https://orcid.org/0000-0002-2089-9055)
- Wenlan Chen (ORCID: https://orcid.org/0000-0003-1521-5365)
- B. S. Yang (ORCID: https://orcid.org/0009-0008-5942-316X)
- Yaoying Long (ORCID: https://orcid.org/0000-0003-4517-1375)
- Si‐Yi Chen (ORCID: https://orcid.org/0009-0009-1384-963X)
- Anyuan Zhang (ORCID: https://orcid.org/0000-0001-7854-7071)
- Wenxiang Ren
- Liuyue Xu
- Di Wu
- Guifen Fan (ORCID: https://orcid.org/0000-0002-0262-0292)
- Qiubai Li (ORCID: https://orcid.org/0000-0001-7884-0745)
- Li Chen (ORCID: https://orcid.org/0000-0001-7670-8062)
- Shan Wang (ORCID: https://orcid.org/0000-0003-3314-6455)
- Ting Chen (ORCID: https://orcid.org/0000-0003-1725-7956)
- Hongxiang Wang (ORCID: https://orcid.org/0000-0002-2499-3604)
- Zhichao Chen (ORCID: https://orcid.org/0000-0002-9884-0002)
- Yulin Cao (ORCID: https://orcid.org/0000-0002-2933-7796)
- Li He (ORCID: https://orcid.org/0000-0003-2289-4888)
- Yali Yu
Institutions
- Union Hospital (HK)
- Army Medical University (CN)
- Hubei University of Science and Technology (CN)
- Southwest Hospital (CN)
- Central Hospital of Wuhan (CN)
- Institute of Advanced Manufacturing Technology (CN)
- Huazhong University of Science and Technology (CN)
Publication Details
- Journal
- ACS Applied Materials & Interfaces
- Published
- 2024-11-06
- DOI
- https://doi.org/10.1021/acsami.4c12725
- Citations
- 4
- Primary Topic
- 3D Printing in Biomedical Research
- Type
- article
- Field-Weighted Citation Impact
- 0.74
Funders
- Health Commission of Hubei Province
- National Natural Science Foundation of China
- Foundation for Innovative Research Groups of Hubei Province of China
- National Key Research and Development Program of China