Differences in the expression of microRNAs 16-5p and 451a after acute exercise in patients with thrombosis versus healthy subjects

<bold>Introduction:</bold> In a previous study we found dysregulation of microRNAs (miRNAs) 16-5p and 451a before and after exercise in patients with perfusion defects after pulmonary embolism (PE). We found and association between levels of these miRNAs and cardiopulmonary exercise testing (CPET) parameters [peak oxygen consumption per mL, Kg and minute (VO2max/ml_kg_min) and ventilatory equivalent of CO2 at the anaerobic threshold (VE/VCO2 at AT)] Aim: To identify if these observations are specific of PE patients. <bold>Methods:</bold> Patients with persistent perfusion defects after PE and healthy subjects carried out a CPET. Blood samples were drawn pre- and post- exercise and miRNAs were quantified. Mixed linear regression models have been adjusted [response variable: miRNA levels, predictor variables: CPET variables, time (pre- or post- exercise) and group (PE or healthy). We evaluated interactions between CPET variables and group, and between the time and group. <bold>Results:</bold> 15 patients and 20 healthy subjects were analyzed. A significant interaction is observed between the time (pre- post-) and the group. We also found a significant interaction between VO2 max/ml_kg_min and group in the case of miR 16-5p (p = 0.023, Figure 1). This interaction does not become significant for VO2 max/ml_kg_min and miR 451a (p = 0.105). <bold>Conclusion:</bold> our observations support PE development affects miR-16-5p expression. <fig><object-id>erj;64/suppl_68/PA535/F1</object-id><object-id>F1</object-id><object-id>F1</object-id><graphic></graphic></fig>