Smoke-Triggered Cell Apoptosis: Role of miRNA200a-ZEB2 Interaction in BEAS-2B Cells

<bold>Objective:</bold> Investigating miRNA200a's role in smoke-induced apoptosis in BEAS-2B cells. <bold>Methods:</bold> Cells exposed to smoke medium were assessed for miRNA200a expression in Control and various CSE concentrations. RT-PCR measured miRNA200a expression post-transfection with miRNA inhibitors and mimics under CSE intervention. Apoptosis was detected via Tunel assay, flow cytometry, and Western Blot. TargetScan predicted miRNA200a-3p binding sites on ZEB2 3'UTR, validated using dual luciferase reporter assay. <bold>Results:</bold> Higher miRNA200a expression observed in CSE-exposed cells, correlating with concentration. Post-inhibitor transfection, 5% CSE intervention reduced miRNA200a expression, while mimics increased it. Inhibitor-treated cells showed reduced apoptosis and altered protein expression. Conversely, mimics increased apoptosis and exhibited opposite protein expression (Fig. 1). TargetScan predicted and validated miRNA200a-3p binding sites on ZEB2 3'UTR (Fig. 2). <bold>Conclusion:</bold> miRNA200a promotes smoke-induced apoptosis in BEAS-2B cells, while miR-200a-3p negatively regulates ZEB2 3'UTR. This study illuminates miRNA200a's role and offers potential therapeutic avenues for smoke-induced lung damage mitigation <fig><object-id>erj;64/suppl_68/PA4386/F1</object-id><object-id>F1</object-id><object-id>F1</object-id><graphic></graphic></fig>