On-site visual detection of Nipah virus combining a reverse transcription recombinase-aided amplification with a lateral-flow dipstick assay1
• The RT-RAA-VF assay developed for the NiV P gene can perform rapid detection of NiV within 20 min at 42°C with high specificity. • This assay is capable of attaining sensitivity to a single copy of NiV RNA transcripts. • This assay effectively avoids false positives caused by aerosol contamination with a sealed disposable nucleic acid visualization test paper device.
Authors
- Kuijuan Jin (ORCID: https://orcid.org/0000-0002-0047-4375)
- Yongsai Liu
- Zimo Zhang
- Lin Ai (ORCID: https://orcid.org/0000-0001-7502-7935)
- Pei Huang (ORCID: https://orcid.org/0000-0001-8012-7105)
- Hao Li (ORCID: https://orcid.org/0000-0001-7191-5118)
- Yuanyuan Li (ORCID: https://orcid.org/0000-0002-2986-588X)
- Junjie Zhao (ORCID: https://orcid.org/0000-0002-0105-0531)
- Zengguo Cao
- Hualei Wang (ORCID: https://orcid.org/0000-0001-6961-1991)
- Huanxin Chen
- Haili Zhang (ORCID: https://orcid.org/0000-0001-8916-069X)
- Yufei Liu (ORCID: https://orcid.org/0000-0003-0441-0528)
- Zanheng Huang
- C. Fan
Institutions
- Jilin University (CN)
- Chinese Academy of Sciences (CN)
- Wuhan Institute of Virology (CN)
- China Institute of Veterinary Drug Control (CN)
- Tsinghua University (CN)
Publication Details
- Journal
- Journal of Integrative Agriculture
- Published
- 2024-11-01
- DOI
- https://doi.org/10.1016/j.jia.2024.11.018
- Citations
- 1
- Primary Topic
- Virology and Viral Diseases
- Type
- article
- Field-Weighted Citation Impact
- 0.39