Wood hemicelluloses as protective materials for preserving the viability of probiotic Lacticaseibacillus rhamnosus GG during spray drying

Wood hemicelluloses from forest industry side-streams are promising economic and sustainable alternatives for encapsulating bioactive compounds. This study explores their suitability for probiotic encapsulation, specifically for maintaining cell viability and structure. The ability of galactoglucomannans (GGM) and glucuronoxylans (GX) to support the survival of Lacticaseibacillus rhamnosus GG (LGG) during spray drying at solid feed concentrations of 15 and 20 % and inlet air temperatures of 105 and 140 °C (outlet air temperature of 50 °C) was investigated and compared to the results obtained using maltodextrin (MD). Across all investigated conditions, LGG survival rates exceeded 85 % (>10⁷ cfu/g) in GX and GGM microcapsule powders, which similar to that in MD microcapsules despite the differences in pH, particle size, and viscosity of their feed dispersions. The GX microcapsules demonstrated the highest process yield (50-58 %), followed by MD (39-51 %). All the microcapsule powders exhibited an amorphous structure consisting of spherical particles with an average diameter of 10 μm, sufficient for LGG accommodation. Atomic force microscopy analysis confirmed the encapsulation of LGG cells within microcapsules with intact rod-shaped chains post-spray drying. Overall, the spray-dried microencapsulation of probiotics using wood hemicelluloses maintains high probiotic viability and offers an eco-friendly, cost-effective alternative to traditional materials.