Microfluidic-based fluorescence enhancement of silica-embedded carbon dots for direct detection and quantification of unamplified HCV RNA in clinical samples
This application of N-CDs/SiO2 as nucleic acid fluorescent probes for the first time, offers a versatile, cost-effective, and universal platform for nucleic acids detection. The developed system can be employed in conventional microwell plate-based detection with minimal modifications to current laboratory setups. Additionally, it was integrated into a 3D-printed microfluidic chip enabling enhancement in specificity, sensitivity and accuracy.
Authors
- El‐Zeiny M. Ebeid (ORCID: https://orcid.org/0000-0002-5830-6470)
- Sherif M. Shawky (ORCID: https://orcid.org/0000-0001-5020-5563)
- Hesham A. Elghazaly (ORCID: https://orcid.org/0000-0002-9327-286X)
- Mohmed K. Hassan
- Mohamed Sharafeldin (ORCID: https://orcid.org/0000-0003-0618-945X)
- Sara H. A. Agwa (ORCID: https://orcid.org/0000-0002-2382-0189)
- Hanan Shaat (ORCID: https://orcid.org/0009-0006-6283-9137)
- Amany Mostafa (ORCID: https://orcid.org/0000-0003-4013-0742)
- Eman H Ismail (ORCID: https://orcid.org/0000-0002-2534-9888)
- Mohamed H. Alkordi (ORCID: https://orcid.org/0000-0002-5965-7897)
Institutions
- Ain Shams University (EG)
- Zagazig University (EG)
- Tanta University (EG)
- National Research Centre (EG)
- Zewail City of Science and Technology (EG)
- Port Said University (EG)
Publication Details
- Journal
- Analytica Chimica Acta
- Published
- 2024-11-07
- DOI
- https://doi.org/10.1016/j.aca.2024.343396
- Citations
- 2
- Primary Topic
- Carbon and Quantum Dots Applications
- Type
- article
- Field-Weighted Citation Impact
- 0.23