Amazonins: New Peptaibol Sequences from an Endophytic Strain of <i>Trichoderma amazonicum</i>
Three new putative sequences of 14-residue peptaibols, named amazonins I-III were characterized from the endophytic fungus Trichoderma amazonicum via genome mining, high-performance liquid chromatography coupled to high-resolution tandem mass spectrometry (LC-MS/MS), and molecular networking. Bioinformatic analysis of the T. amazonicum genome assembly revealed 63 clusters of biosynthetic genes (BGCs) related to secondary metabolites, including a nonribosomal peptide synthetase accountable for the biosynthesis of the discovered peptide sequences. Analysis of the adenylation domains, along with manual interpretation of MS/MS spectra, allowed extensive annotation of the new peptaibol sequences. The combination of bioinformatic tools and LC-MS/MS provides a better opportunity to characterize and identify new peptaibol sequences. Thus, the importance of studies on the production and characterization of peptaibols produced by Trichoderma species from the Amazon region is highlighted.
Authors
- Gleucinei dos Santos Castro (ORCID: https://orcid.org/0000-0002-0316-5949)
- Ingride Jarline Santos da Silva (ORCID: https://orcid.org/0000-0002-8726-226X)
- Thiago Fernandes Sousa
- Evelyn Maribel Condori Peñaloza (ORCID: https://orcid.org/0000-0001-7275-2816)
- Rafael Garrett (ORCID: https://orcid.org/0000-0002-5823-311X)
- Gilvan Ferreira da Silva (ORCID: https://orcid.org/0000-0003-2828-8299)
- D Raposo (ORCID: https://orcid.org/0009-0005-9412-9378)
- M. E. B. Yamagishi (ORCID: https://orcid.org/0000-0001-7109-3038)
- Héctor H. F. Koolen (ORCID: https://orcid.org/0000-0002-0181-348X)
- José Carlos Ipuchima da Silva (ORCID: https://orcid.org/0000-0002-3628-7985)
Institutions
- Universidade Federal do Rio de Janeiro (BR)
- Brazilian Agricultural Research Corporation (BR)
- Universidade do Estado do Amazonas (BR)
- Universidade Federal do Amazonas (BR)
Publication Details
- Journal
- Chemistry & Biodiversity
- Published
- 2024-11-04
- DOI
- https://doi.org/10.1002/cbdv.202400611
- Citations
- 2
- Primary Topic
- Genomics and Phylogenetic Studies
- Type
- article
- Field-Weighted Citation Impact
- 0.47