A highly contiguous genome sequence of Alternaria porri isolate Apn-Nashik causing purple blotch disease in onion
Long-read sequencing on a PacBio Sequel II system resulted in a 32.98 Mb (20 contigs) assembly with an N50 of 2, 657, 264 bp, the longest contig length of 5.05 Mb, and a GC content of 51.06%. The Benchmarking Universal Single-Copy Orthologs (BUSCO) analysis resulted in 99.7% genome completeness at the Dothideomycetes lineage, representing a high-quality genome assembly. AUGUSTUS ab initio analysis resulted in 9875 protein-coding genes. Of the 6776 pathogenicity-related genes, 537 genes with effector functions were identified. Likewise, the glycoside hydrolases (434) were the most dominant group of the total 837 predicted CAZymes. The assembled genome of A. porri showed distinctive similarities to the genomes of A. alternata and A. brassicicola, the causal agents of leaf blight of onion and leaf spot of Brassica crops, respectively.
Authors
- Rukmini Mishra (ORCID: https://orcid.org/0000-0001-9692-212X)
- Raj Kumar Joshi (ORCID: https://orcid.org/0000-0003-0505-2881)
- Richa Sharma (ORCID: https://orcid.org/0000-0001-9747-0700)
Institutions
- Rama Devi Women's University (IN)
- Centurion University of Technology and Management (IN)
Publication Details
- Journal
- BMC Genomic Data
- Published
- 2024-11-05
- DOI
- https://doi.org/10.1186/s12863-024-01276-0
- Citations
- 2
- Primary Topic
- Fungal Plant Pathogen Control
- Type
- article
- Field-Weighted Citation Impact
- 2.06
Funders
- Department of Biotechnology, Ministry of Science and Technology, India
- Department of Science and Technology, Ministry of Science and Technology, India
- University Grants Commission
- Department of Biotechnology, Government of West Bengal