Spatial variability of soil microbiological properties under different land use systems

Understanding the spatial variability of soil microbial properties is crucial for assessing their role in nutrient cycling and ecosystem functioning across various land use systems. While many studies emphasize soil physicochemical characteristics, the spatial heterogeneity of microbiological attributes is less explored. This research examined the spatial distribution of microbial biomass carbon (MBC) and dehydrogenase activity (DHA) in surface soils of berseem, rice-wheat, and poplar-wheat cropping systems. Forty-eight georeferenced surface soil samples (0-0.15 m) were collected from each system and analyzed for MBC, DHA, and physicochemical properties. Sensitivity analysis determined the minimum sample size needed for effective sampling. Results showed strong spatial dependence for the berseem and poplar-wheat systems. Principal component analysis (PCA) and discriminant analysis (DA) identified MBC, DHA, and soil organic carbon (SOC) as key variables distinguishing the land use systems. Soil microbiological characteristics exhibited greater variability than chemical properties, with DHA showing more variability than MBC, necessitating larger sample sizes to detect changes. This study highlights the importance of understanding spatial variation in soil microbial properties for
\ndesigning sampling protocols and implementing sustainable management practices, ultimately enhancing ecosystem functioning and nutrient cycling in diverse agricultural systems.