Quantitative analysis of sinigrin from deoiled cake in different Brassica species genotypes

Oil seeds play a great role in human and animal nutrition. Brassica is an agriculturally important genus containing species with highly diverse morphology and wide ranging utility. Rapseed-Mustard meal is highly regarded as a component of animal feed with high protein content and a desirable amino acid profile. The presence of some components, in particular glucosinolates (sinigrin) affects the value of the meal and reduces the amount that can be used in animal feed formulations. Quantitative analysis of sinigrin was done in thirty genotypes of Brassica species. All the genotypes were collected from the Oilseeds Research Centre of S. D. Agricultural University, Sardarkrushinagar for High performance liquid chromatography (HPLC) analysis. Significant variation was found among the genotypes. Concentration range of sinigrin in deoiled cake of thirty genotypes was from 2.92 µmol/g DM (RTM314) to 173.14 µmol/g DM (NRCG35). Genotypes of Brassica juncea were all genotypes sowed <30 µmol/g while Brassica napus NRCG35 genotype sowed highest sinigrin content 173.14 µmol/g. The Brassica juncea species and other species genotypes which had low content of sinigrin where all genotypes good for animal feed and used for the double zero varieties by breeding program. The glucosinolates have also been applied in the prevention of bacterial and fungal spoilage of food products in advanced atmospheric packaging technology which improves the shelf-life of these products. These issues are important priorities for rapeseed mustard breeders.