Precision-Cut Lung Slices: Emerging Tools for Preclinical and Translational Lung Research: An Official American Thoracic Society Workshop Report
The urgent need for effective treatments for acute and chronic lung diseases underscores the significance of developing innovative preclinical human research tools. The 2023 American Thoracic Society Workshop on Precision-Cut Lung Slices (PCLSs) brought together 35 experts to discuss and address the role of human tissue-derived PCLSs as a unique tool for target and drug discovery and validation in pulmonary medicine. With increasing interest and usage, together with advancements in methods and technology, there is a growing need for consensus on PCLS methodology and readouts. The current document recommends standard reporting criteria and emphasizes the requirement for careful collection and integration of clinical metadata. We further discuss current clinically relevant readouts that can be applied to PCLSs and highlight recent developments and future steps for implementing novel technologies for PCLS modeling and analysis. The collection and correlation of clinical metadata and multiomic analysis will further advance the integration of this preclinical platform into patient endotyping and the development of tailored therapies for patients with lung disease.
Authors
- Gloria Pryhuber (ORCID: https://orcid.org/0000-0002-9185-3994)
- Anna Zagórska (ORCID: https://orcid.org/0000-0003-3703-1364)
- Christopher D. Anderson (ORCID: https://orcid.org/0000-0002-0053-2002)
- Hrishikesh S. Kulkarni (ORCID: https://orcid.org/0000-0003-4830-5084)
- Ramaswamy Krishnan (ORCID: https://orcid.org/0000-0003-4994-0709)
- Scott Turner (ORCID: https://orcid.org/0000-0002-6618-6405)
- Charlotte H. Dean (ORCID: https://orcid.org/0000-0002-8846-5472)
- Katherina Sewald (ORCID: https://orcid.org/0000-0002-7804-4527)
- Ganesh Raghu (ORCID: https://orcid.org/0000-0001-7506-6643)
- Jordan P. Metcalf (ORCID: https://orcid.org/0000-0003-4359-5270)
- Mareike Lehmann (ORCID: https://orcid.org/0000-0002-8601-8206)
- Ashish Ranjan Sharma (ORCID: https://orcid.org/0000-0003-3973-6755)
- Iván O. Rosas (ORCID: https://orcid.org/0000-0003-4622-618X)
- Enid Neptune (ORCID: https://orcid.org/0000-0002-9635-1272)
- Bélâ Suki (ORCID: https://orcid.org/0000-0002-9720-1006)
- Scott H. Randell (ORCID: https://orcid.org/0000-0001-5351-2841)
- Amanda L. Tatler (ORCID: https://orcid.org/0000-0002-4644-4614)
- Pam Van Ry
- Jane E. Bourke (ORCID: https://orcid.org/0000-0001-7314-9234)
- Andrew J. Haak
- Claudia Loebel (ORCID: https://orcid.org/0000-0002-3140-5663)
- Cynthia Koziol‐White (ORCID: https://orcid.org/0000-0003-1101-2747)
- Timo Wille (ORCID: https://orcid.org/0000-0002-4359-5388)
- Jennifer M. S. Sucre (ORCID: https://orcid.org/0000-0002-6613-1439)
- Mélanie Königshoff (ORCID: https://orcid.org/0000-0001-9414-5128)
- Holger Behrsing
- Alexandra Noël (ORCID: https://orcid.org/0000-0001-5634-8151)
- Anne I. Sperling (ORCID: https://orcid.org/0000-0002-4265-9212)
- Naftali Kaminski (ORCID: https://orcid.org/0000-0001-5917-4601)
- Nicholas E. Banovich (ORCID: https://orcid.org/0000-0003-2604-3247)
- Yong Ho Kim (ORCID: https://orcid.org/0000-0001-7527-0134)
- Mauricio M. Rojas (ORCID: https://orcid.org/0000-0001-7340-4784)
- Ricardo Pineda (ORCID: https://orcid.org/0000-0001-8768-4801)
- Reinoud Gosens
Institutions
- Brigham Young University (US)
- Rutgers, The State University of New Jersey (US)
- Boston University (US)
- University of North Carolina at Chapel Hill (US)
- Louisiana State University (US)
- Brigham and Women's Hospital (US)
- Translational Genomics Research Institute (US)
- Environmental Protection Agency (US)
- Research Triangle Park Foundation (US)
- Beth Israel Deaconess Medical Center (US)
- Mayo Clinic (US)
- University of Nottingham (GB)
- Johns Hopkins University (US)
- Philipps University of Marburg (DE)
- University of Groningen (NL)
- University of Pittsburgh (US)
- Washington University in St. Louis (US)
- University of Michigan (US)
- Johns Hopkins Medicine (US)
- Australian Regenerative Medicine Institute (AU)
- University of Washington Medical Center (US)
- Helmholtz Zentrum München (DE)
- Yale University (US)
- University of Florida (US)
- Institute for In Vitro Sciences (US)
- Fraunhofer Institute for Toxicology and Experimental Medicine (DE)
- Pliant (United States) (US)
- Gilead Sciences (United States) (US)
- Bundeswehr Medical Service Academy (DE)
- Pulmonary and Critical Care Associates (US)
- Institut für Pharmakologie und Toxikologie der Bundeswehr
- Imperial College London (GB)
- University of Oklahoma Health Sciences Center (US)
- University of Virginia (US)
- The Ohio State University (US)
- University of Rochester (US)
- Monash University (AU)
- Vanderbilt University Medical Center (US)
Publication Details
- Journal
- American Journal of Respiratory Cell and Molecular Biology
- Published
- 2024-11-05
- DOI
- https://doi.org/10.1165/rcmb.2024-0479st
- Citations
- 27
- Primary Topic
- Lung Cancer Treatments and Mutations
- Type
- article
- Field-Weighted Citation Impact
- 11.31
Funders
- U.S. Environmental Protection Agency
- U.S. Department of Veterans Affairs
- Presbyterian Health Foundation
- American Thoracic Society
- Jain Foundation
- Bristol-Myers Squibb
- Pfizer
- AstraZeneca
- Gilead Sciences
- FibroGen
- Ohio State University
- F. Hoffmann-La Roche
- United Therapeutics Corporation
- University of Pittsburgh
- University of Rochester
- CSL Behring
- European Respiratory Society
- Veracyte
- Moderna
- Asthma and Lung UK
- Deutsche Forschungsgemeinschaft
- State Government of Victoria
- Lung Foundation Australia
- Deutsches Zentrum für Lungenforschung
- Universitas Hasanuddin
- National Institutes of Health
- Directorate for Biological Sciences
- Genentech
- Novartis Pharma
- AbbVie Biotherapeutics
- Medical Research Council
- National Health and Medical Research Council
- National Heart, Lung, and Blood Institute
- National Institute of General Medical Sciences