Construction of multilayered gene circuits using de-novo-designed synthetic transcriptional regulators in cell-free systems
We demonstrated a practical strategy for designing standardized regulatory elements and assembling modular gene circuits, ultimately laying the foundation for the streamlined construction of complex synthetic gene circuits.
Authors
- Jinzhen Jiao (ORCID: https://orcid.org/0000-0002-6064-2270)
- Shaobin Guo (ORCID: https://orcid.org/0000-0001-9736-4078)
- Jongmin Kim (ORCID: https://orcid.org/0000-0002-2713-1006)
- Jeongwon Kim (ORCID: https://orcid.org/0000-0002-5683-3613)
- Mingming Zhao
- Xianai Shi (ORCID: https://orcid.org/0000-0002-1897-2786)
- Yelin Lim
Institutions
- Pohang University of Science and Technology (KR)
- Fujian Medical University (CN)
- Fuzhou University (CN)
Publication Details
- Journal
- Journal of Biological Engineering
- Published
- 2024-11-05
- DOI
- https://doi.org/10.1186/s13036-024-00459-8
- Citations
- 3
- Primary Topic
- CRISPR and Genetic Engineering
- Type
- article
- Field-Weighted Citation Impact
- 0.70
Funders
- National Research Foundation
- National Natural Science Foundation of China
- Pohang University of Science and Technology
- Natural Science Foundation of Fujian Province
- Ministry of Agriculture, Food and Rural Affairs
- Korea Health Industry Development Institute
- Korea Basic Science Institute
- National Research Foundation of Korea
- Ministry of Science and ICT, South Korea
- Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry