A magnetic epitope-imprinted microsphere used for selective separation and rapid detection of SHV-type β-lactamases in bacteria: a novel strategy of antimicrobial resistance detection
This enzyme-specific detection strategy combined the specificity of epitope imprinting with the sensitivity of mass spectrometry, enabling β-lactamase to be selectively extracted from bacteria and clearly presented in mass spectra. Compared with other drug resistance detection methods, this technique has good specificity, high sensitivity (≤ 15 mg of bacteria), a short MTTD (less than 2 h), and simple operation, and therefore has a broad application prospect in clinical medicine.
Authors
- Yusun Zhou (ORCID: https://orcid.org/0000-0001-8618-3853)
- Kanming Wang (ORCID: https://orcid.org/0000-0002-3762-3274)
- Yuanyuan Chi
- Lele Li (ORCID: https://orcid.org/0000-0001-8593-9292)
- Baosheng Ge (ORCID: https://orcid.org/0000-0001-9906-2081)
- Hui Li (ORCID: https://orcid.org/0000-0002-9312-1607)
- Qing Wang (ORCID: https://orcid.org/0000-0003-1295-1930)
- Shu-Hui Liu (ORCID: https://orcid.org/0000-0002-1540-6930)
- Xiaotong Xu (ORCID: https://orcid.org/0009-0006-1883-6692)
- Bing Yu
- Tingting Zhou (ORCID: https://orcid.org/0000-0001-5782-8456)
- Meng Han (ORCID: https://orcid.org/0000-0002-6219-2656)
- Yuanqi Zhu
- Qingwu Tian
Institutions
- Qingdao University (CN)
- Affiliated Hospital of Qingdao University (CN)
- China University of Petroleum, East China (CN)
- Qingdao Women and Children's Hospital (CN)
- Zhejiang University (CN)
Publication Details
- Journal
- Journal of Nanobiotechnology
- Published
- 2024-11-05
- DOI
- https://doi.org/10.1186/s12951-024-02949-9
- Citations
- 4
- Primary Topic
- Antibiotic Resistance in Bacteria
- Type
- article
- Field-Weighted Citation Impact
- 0.68
Funders
- China Postdoctoral Science Foundation
- China University of Petroleum, Beijing
- Natural Science Foundation of Shandong Province
- Qingdao University