The Effect of Increasing Aerobic Exercise on the Expression of NF-κB and COX-2 Genes in Heart Tissue of Rats Under a High-fat Diet

Background: Several studies have shown that obesity alters the function and structure of the heart. Objectives: This study examined the impact of increasing aerobic exercise (IAE) on the mRNA expression of Cyclooxygenase-2 (COX-2) and nuclear factor Kappa B (NF-κB) in the cardiac tissue of rats fed a high-fat diet (HFD). Methods: Twenty-four male Wistar rats were divided into two groups and fed two types of diets for eight weeks: Control and HFD groups. After eight weeks, the rats on the HFD were further divided into HFD and HFD + IAE groups. The exercise program was conducted for six weeks, with five sessions per week, progressing from moderate to high intensity. The program followed the principle of gradual overload and was based on the maximum speed of the rats. The expression of COX-2 and NF-κB genes was assessed. Results: The expression of COX-2 and NF-κB genes increased in the HFD group compared to the control group. After six weeks, the expression of NF-κB and COX-2 genes decreased in the HFD + IAE group compared to the HFD group. Conclusions: Six weeks of IAE appears to reduce the inflammatory process in rats with HFD-induced obesity.