Improvement of the Sand Quality by Applying Microorganism Induced Calcium Carbonate Precipitation to Reduce Cement Usage

This research aimed to study the utilization of microorganisms in the soil. These microorganisms were applied to improve the quality of soil and sand to reduce cement consumption. The process of microbial-induced precipitation of calcium carbonate (MICP) was used in this study, a bonding process to create a binding between sand particles from the naturally occurring processes of microorganisms. The study was conducted to determine the maximum growth rate of bacteria (maximum growth curve) of five strains as follows Proteus mirabilis TISTR 100, Bacillus thuringiensis TISTR 126, Staphylococcus aureus TISTR 118, Bacillus sp. TISTR 658 and Bacillus megaterium TISTR 067 in Nutrient Broth (NB) culture medium at 37°C. Christensen's Urea Agar slant (UA) was a preliminary screen for urease-producing bacteria. The color change of indicator color was seen in the slant, comparing the results (+) and control (-). The researchers have selected bacterial strains capable of producing significant amounts of enzymes to hydrolyze urea. It was found that the amount of calcium carbonate sediment produced by five strains of bacteria, namely Proteus mirabilis TISTR 100, Bacillus thuringiensis TISTR 126, Staphylococcus aureus TISTR 118, Bacillus sp. TISTR 658 and Bacillus megaterium TISTR 067 had the amount of sediment produced as follows: 3.430, 3.080, 2.590, 1.985, and 1.615 mg/ml, respectively. It was found that the species Bacillus sp. TISTR 658 does not produce the most calcium carbonate sediment. Nevertheless, it made the sand samples stick together tightly and form the perfect lumps. Therefore, improving the sand's composition has shown the potential to apply the MICP process to reduce the cement used.