Inheritance and Fitness Cost of Laboratory-Selected Resistance to Cry1Ab in Hyphantria cunea Drury (Lepidoptera: Arctiidae)
Transgenic poplars have been used to control quarantine pests worldwide, such as the fall webworm (<i>Hyphantria cunea</i>, FW). However, the studies on the resistance mechanism of FW to Cry toxins are limited. This study obtained an FW strain with 45-fold resistance to Cry1Ab toxin by continuous screening in the laboratory. The resistance to Cry1Ab was autosomally completely recessive, and it had approximately 40-fold cross-resistance to Cry1Ac but no cross-resistance to Cry2Ab toxin. Analysis of indoor population life tables for the resistant and susceptible strains found no significant difference in the net reproduction rate (R<sub>0</sub>) between the two strains. The resistant strain had an insignificantly longer larval development duration and a comparable pupation rate, pupal weight, emergence rate, eggs laidper female, and egg hatching rate compared to the susceptible strain. This indicated that there was no fitness cost for Cry1Ab resistance. Our study helps in evaluating the risk of resistance to Cry1Ab toxin and the mechanisms of resistance to Cry1Ab toxin in FW.
Authors
- Laipan Liu (ORCID: https://orcid.org/0000-0002-7383-8096)
- Xin Yin (ORCID: https://orcid.org/0000-0002-4759-0226)
- Zhixiang Fang (ORCID: https://orcid.org/0000-0003-1651-878X)
- Zhentao Ren (ORCID: https://orcid.org/0000-0003-0468-2013)
- Li Zhang (ORCID: https://orcid.org/0009-0006-5375-8458)
- Biao Liu (ORCID: https://orcid.org/0000-0002-4430-5426)
- Wenjing Shen
- Yu Qi (ORCID: https://orcid.org/0009-0005-7785-4324)
Institutions
- Nanjing Institute of Environmental Sciences (CN)
Publication Details
- Journal
- Insects
- Published
- 2024-11-04
- DOI
- https://doi.org/10.3390/insects15110861
- Primary Topic
- Insect Resistance and Genetics
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Government of Jiangsu Province
- Natural Science Foundation of Jiangsu Province