Color and Spatial Frequency Provide Functional Signatures of Retinotopic Visual Areas

Primate vision relies on retinotopically organized cortical parcels defined by representations of hemifield (upper vs lower visual field), eccentricity (fovea vs periphery), and area (V1, V2, V3, V4). Here we test for functional signatures of these organizing principles. We used functional magnetic resonance imaging to measure responses to gratings varying in spatial frequency, color, and saturation across retinotopically defined parcels in two macaque monkeys, and we developed a Sparse Supervised Embedding (SSE) analysis to identify stimulus features that best distinguish cortical parcels from each other. Constraining the SSE model to distinguish just eccentricity representations of the voxels revealed the expected variation of spatial frequency and S-cone modulation with eccentricity. Constraining the model according to the dorsal/ventral location and retinotopic area of each voxel provided unexpected functional signatures, which we investigated further with standard univariate analyses. Posterior parcels (V1) were distinguished from anterior parcels (V4) by differential responses to chromatic and luminance contrast, especially of low-spatial-frequency gratings. Meanwhile, ventral parcels were distinguished from dorsal parcels by differential responses to chromatic and luminance contrast, especially of colors that modulate all three cone types. The dorsal/ventral asymmetry not only resembled differences between candidate dorsal and ventral subdivisions of human V4 but also extended to include all retinotopic visual areas, starting in V1 and increasing from V1 to V4. The results provide insight into the functional roles of different retinotopic areas and demonstrate the utility of SSE as a data-driven tool for generating hypotheses about cortical function and behavior.