Expanding the landscape of systemic sclerosis-related autoantibodies through RNA immunoprecipitation coupled with massive parallel sequencing
A novel RIP-Seq assay has been developed to detect autoantibodies targeting ribonucleoprotein complexes in SSc patients. This method successfully identified RNA molecules associated with ribonucleoproteins known to be targeted by SSc-related autoantibodies, validating both the assay and the analysis strategy. Additionally, this approach uncovered RNA molecules associated with ribonucleoproteins that were not previously identified as targets of SSc patients' sera, suggesting potential new autoantibody candidates in this disease.
Authors
- Albert Selva-O’Callaghan
- Laura Viñas-Giménez (ORCID: https://orcid.org/0000-0003-2913-1154)
- Fernando Gordillo-González
- Laura Batlle-Masó (ORCID: https://orcid.org/0000-0002-4209-176X)
- Maria Teresa Sanz-Martínez (ORCID: https://orcid.org/0000-0001-5814-8698)
- Roger Colobrán
- Ricardo Pujol‐Borrell (ORCID: https://orcid.org/0000-0001-7833-675X)
- L. Triginer
- Javier J. Martin (ORCID: https://orcid.org/0000-0002-2202-0622)
- Neil McHugh (ORCID: https://orcid.org/0000-0003-2765-658X)
- Eduardo Andrés‐León (ORCID: https://orcid.org/0000-0002-0621-9914)
- Sarah Tansley (ORCID: https://orcid.org/0000-0003-4633-9598)
- Janire Perurena-Prieto (ORCID: https://orcid.org/0000-0002-3048-1058)
- Alfredo Castillo (ORCID: https://orcid.org/0000-0003-0626-507X)
- Carmen Pilar Simeón‐Aznar (ORCID: https://orcid.org/0000-0003-3390-9029)
- Claudia Codina-Clavaguera (ORCID: https://orcid.org/0000-0002-8083-5897)
Institutions
- Hebron University (PS)
- Universitat Autònoma de Barcelona (ES)
- Vall d'Hebron Institut de Recerca (ES)
- Vall d'Hebron Hospital Universitari (ES)
- Instituto de Parasitología y Biomedicina "López - Neyra" (ES)
- University of Bath (GB)
Publication Details
- Journal
- Journal of Autoimmunity
- Published
- 2024-11-04
- DOI
- https://doi.org/10.1016/j.jaut.2024.103328
- Citations
- 2
- Primary Topic
- Systemic Sclerosis and Related Diseases
- Type
- article
- Field-Weighted Citation Impact
- 0.85