ANTIBIOFILM ACTIVITY OF PROBIOTIC LACTIC ACID BACTERIA FROM COCKROACH (PERIPLANETA AMERICANA) GUT

This study has been focused on Antibiofilm activity of probiotic lactic acid bacteria from cockroach (Periplaneta americana) gut. A total of 20 samples were collected from the cockroach gut isolating bacteria using a 10-fold serial dilution on agar plates incubated anaerobically. Further characterization of the isolated strains included assessing their morphology, Gram staining, and performing various biochemical tests. Isolated strains were evaluated for antimicrobial activity against indicators strains i.e., E. coli, p. aeruginosa, s. aureus and klebsiella. Wider zones of inhibition were observed in the case of LAB isolates against E. coli, with a range extending from 34mm to 20mm. Among 08 LAB strains 03 were resistant to streptomycin and vancomycin. Probiotic characterization included NaCl, pH, and bile salt tolerance, temperature resistance, auto-aggregation, co-aggregation, and cell surface hydrophobicity. Probiotic strains Lactobacillus plantarum CE56.8 and Lacticaseibacillus rhamnosus 5974 showed highest growth rate at 8% NaCl concentration. These strains displayed significant growth under various conditions, indicating their potential as probiotics. Importantly, these strains exhibited a notable ability to inhibit biofilm formation by E. coli (88%) and P. aeruginosa (67%), offer promising effect in combatting biofilm-related infections. explored promising possibilities in the domain of beneficial bacteria, including lactic acid bacteria isolated from the gut of cockroach and lays the foundation for future investigations. However further research is needed to fully understand and develop LAB-based therapeutics for such infections.