Green synthesis of silver nanoparticles from Koso (hagenia abyssinica (bruce)) and Gesho (rhamnus prinoides) leaf extract and characterization and evaluation of their antimicrobial activity

An eco-friendly green mediated synthesis of inorganic nanoparticles is fast-growing research in the limb of nanotechnology. Preparation of silver nanostructures with controlled crystal structure and morphology is, thus, receiving greater attention. In this research project, a facile and eco-friendly method was employed to successfully synthesize silver nanoparticles structure from Koso and Gesho leaf extract and silver nitrate precursor. silver nanoparticles (AgNPs) were prepared by the reaction of 30 ml silver nitrate and 20 ml leaf extract of both types of plants separately. Visually, the formation of silver nanoparticle was confirmed by observing the color changes from yellow to grey black for Gesho and dark red-purple color for Koso and an intense peak was observed in the UV - spectrophotometer at 379 nm and 371 nm respectively. Finally, the synthesized silver nanoparticle shows antimicrobial activity against S. aureus, E. coli and P. aeruginosa.