A hapten design strategy to enhance the selectivity of monoclonal antibodies against malachite green

Malachite green (MG), a triphenylmethane dye, is used in the aquaculture industry as a disinfectant and insect repellent due to its potent bactericidal and pesticidal properties. However, its use poses potential environmental and health risks. This study analyzed and designed two haptens using computer simulation. Serum data confirmed the feasibility of introducing an arm at the dimethylamine group. Subsequently, a highly selective monoclonal antibody strain was successfully prepared based on the hapten. After optimizing the working conditions of indirect competitive enzyme-linked immunosorbent assay (IC-ELISA), the IC 50 value was 0.83 ng/mL, with a detection limit (IC 10 ) of 0.08 ng/mL and a linear range of 0.19–3.52 ng/mL. The developed monoclonal antibody exhibited a crossover rate of less than 0.1% with other similar structures and can be used to establish an immunoassay.

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Publication Details

Journal
Frontiers in Sustainable Food Systems
Published
2024-10-31
DOI
https://doi.org/10.3389/fsufs.2024.1490750
Citations
2
Primary Topic
Monoclonal and Polyclonal Antibodies Research
Type
article
Field-Weighted Citation Impact
0.81

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article

A hapten design strategy to enhance the selectivity of monoclonal antibodies against malachite green

Min-Fu Wu, Wenqi Xiong, Zhenlin Xu, Nuo Xu et al.
2 citations
Frontiers in Sustainable Food Systems
Monoclonal and Polyclonal Antibodies Research
0.81
article

A hapten design strategy to enhance the selectivity of monoclonal antibodies against malachite green

Min-Fu Wu, Wenqi Xiong, Zhenlin Xu, Nuo Xu, Hongtao Lei, Minhua Wu, Xinan Huang, Yan Huang, Yongjun Li, Sha Li, Jiadong Li, Ri-Sheng Chen
article en
2 citations

Abstract

Malachite green (MG), a triphenylmethane dye, is used in the aquaculture industry as a disinfectant and insect repellent due to its potent bactericidal and pesticidal properties. However, its use poses potential environmental and health risks. This study analyzed and designed two haptens using computer simulation. Serum data confirmed the feasibility of introducing an arm at the dimethylamine group. Subsequently, a highly selective monoclonal antibody strain was successfully prepared based on the hapten. After optimizing the working conditions of indirect competitive enzyme-linked immunosorbent assay (IC-ELISA), the IC 50 value was 0.83 ng/mL, with a detection limit (IC 10 ) of 0.08 ng/mL and a linear range of 0.19–3.52 ng/mL. The developed monoclonal antibody exhibited a crossover rate of less than 0.1% with other similar structures and can be used to establish an immunoassay.

Frontiers in Sustainable Food SystemsVol. 8
South China Agricultural University (CN), Guangzhou University of Chinese Medicine (CN), Guangdong Medical College (CN), Guangdong Pharmaceutical University (CN), Guangzhou Vocational College of Science and Technology (CN), Shunde Polytechnic (CN), Chiang Mai University (TH)
Basic and Applied Basic Research Foundation of Guangdong Province
Responsible consumption and production
Openalex Percentile: Top 25%
Monoclonal and Polyclonal Antibodies Research
0.81
DOIOpen Access PDFOpenAlex
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