<scp><i>PbRVE6</i></scp> Promotes Anthocyanins Accumulation in Pear Fruit Peel by Regulating Key Biosynthetic Genes

Red peel color, a desirable trait in pears, is determined by anthocyanin accumulation. While REVEILLE (RVEs) transcription factors regulate anthocyanin biosynthesis in some plant species, their role in pear peel has not been well-studied. This study investigates the function of RVEs in anthocyanin biosynthesis in 'Zaosu' (low anthocyanin content) and its red bud mutant, 'Red Zaosu' (high anthocyanin content) fruit peel. Consistent with higher anthocyanin content, 'Red Zaosu' pears exhibited increased PbRVE6 expression compared to 'Zaosu', while PbRVE3a and PbRVE3b levels remained unchanged. Additionally, PbRVE6 was localized to the nucleus. Overexpression of PbRVE6 in 'Zaosu' pear pericarp significantly increased anthocyanin content and upregulated key anthocyanin pathway genes PbANS and PbUFGT. Conversely, VIGS silencing of PbRVE6 in 'Red Palacer' pears led to decreased expression of PbANS and PbUFGT and a slight reduction in anthocyanin content. Yeast one-hybrid and dual-luciferase assays confirmed that PbRVE6 can bind and activate the promoters of PbANS and PbUFGT. These findings demonstrate that PbRVE6 promotes anthocyanin accumulation in pear peel by directly regulating PbANS and PbUFGT expression. This study provides a valuable foundation for understanding the regulatory network of anthocyanins in pear peels, and offers potential genetic resources for the production of new pear germplasm.