The Circulating Proteome─Technological Developments, Current Challenges, and Future Trends
Recent improvements in proteomics technologies have fundamentally altered our capacities to characterize human biology. There is an ever-growing interest in using these novel methods for studying the circulating proteome, as blood offers an accessible window into human health. However, every methodological innovation and analytical progress calls for reassessing our existing approaches and routines to ensure that the new data will add value to the greater biomedical research community and avoid previous errors. As representatives of HUPO's Human Plasma Proteome Project (HPPP), we present our 2024 survey of the current progress in our community, including the latest build of the Human Plasma Proteome PeptideAtlas that now comprises 4608 proteins detected in 113 data sets. We then discuss the updates of established proteomics methods, emerging technologies, and investigations of proteoforms, protein networks, extracellualr vesicles, circulating antibodies and microsamples. Finally, we provide a prospective view of using the current and emerging proteomics tools in studies of circulating proteins.
Authors
- Johannes B. Mueller‐Reif (ORCID: https://orcid.org/0000-0003-3454-2396)
- Gilbert S. Omenn (ORCID: https://orcid.org/0000-0002-8976-6074)
- Fredrik Edfors (ORCID: https://orcid.org/0000-0002-0017-7987)
- Laura F. Dagley (ORCID: https://orcid.org/0000-0003-4171-3712)
- Daniel Hornburg (ORCID: https://orcid.org/0000-0002-6618-7774)
- Virginie Brun (ORCID: https://orcid.org/0000-0002-9098-8707)
- Stefanie M. Hauck (ORCID: https://orcid.org/0000-0002-1630-6827)
- Sara Ahadi (ORCID: https://orcid.org/0000-0002-7849-2135)
- Jochen M. Schwenk (ORCID: https://orcid.org/0000-0001-8141-8449)
- Yves Vandenbrouck (ORCID: https://orcid.org/0000-0002-1292-373X)
- Robert L. Moritz (ORCID: https://orcid.org/0000-0002-3216-9447)
- Maria Pernemalm (ORCID: https://orcid.org/0000-0003-4624-031X)
- Xiaobo Yu (ORCID: https://orcid.org/0000-0003-2352-9866)
- Vincent Albrecht (ORCID: https://orcid.org/0009-0003-1985-7733)
- Eric W. Deutsch (ORCID: https://orcid.org/0000-0001-8732-0928)
- Krishnan K. Palaniappan (ORCID: https://orcid.org/0000-0003-0203-9016)
- Zhi Sun (ORCID: https://orcid.org/0000-0003-3324-6851)
- Philipp E. Geyer (ORCID: https://orcid.org/0000-0001-7980-4826)
Institutions
- Centre National de la Recherche Scientifique (FR)
- Inserm (FR)
- The University of Melbourne (AU)
- Bruker (United States) (US)
- Walter and Eliza Hall Institute of Medical Research (AU)
- Commissariat à l'Énergie Atomique et aux Énergies Alternatives (FR)
- University of Michigan (US)
- Science for Life Laboratory (SE)
- Karolinska Institutet (SE)
- Helmholtz Zentrum München (DE)
- Institute for Systems Biology (US)
- Direction de la Recherche Fondamentale (FR)
- Clinatec (FR)
- Direction des Energies (FR)
- CEA Paris-Saclay (FR)
- Freenome (United States) (US)
- Beijing Proteome Research Center (CN)
- Laboratoire d'Électronique des Technologies de l'Information (FR)
- Max Planck Institute of Biochemistry (DE)
- KTH Royal Institute of Technology (SE)
- Université Grenoble Alpes (FR)
Publication Details
- Journal
- Journal of Proteome Research
- Published
- 2024-10-31
- DOI
- https://doi.org/10.1021/acs.jproteome.4c00586
- Citations
- 42
- Primary Topic
- Advanced Proteomics Techniques and Applications
- Type
- article
- Field-Weighted Citation Impact
- 10.11
Funders
- National Science Foundation
- Université Grenoble Alpes
- Institut National de la Santé et de la Recherche Médicale
- Knut och Alice Wallenbergs Stiftelse
- Max-Planck-Gesellschaft
- Kungliga Tekniska Högskolan
- Centre National de la Recherche Scientifique
- Science for Life Laboratory
- National Institutes of Health